Hybridization and complex evolution of Barbarea vulgaris and related species (Brassicaceae)
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Hybridization and complex evolution of Barbarea vulgaris and related species (Brassicaceae). / Lange, Conny Bruun Asmussen; Hauser, Thure Pavlo; Deichmann, Vinnie; Ørgaard, Marian.
In: Molecular Phylogenetics and Evolution, Vol. 169, 107425, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Hybridization and complex evolution of Barbarea vulgaris and related species (Brassicaceae)
AU - Lange, Conny Bruun Asmussen
AU - Hauser, Thure Pavlo
AU - Deichmann, Vinnie
AU - Ørgaard, Marian
N1 - Funding Information: This study was made possible by seeds collected or donated by several persons or institutions over the years, especially Jens Kvist Nielsen and Niels Agerbirk, but also Stina Christensen, Mick Ilowski, Nicole van Dam, Alan Renwick and Francis Chew. Niels Agerbirk commented on an earlier version of this manuscript, and Pernille Ø. Erthmann helped with retrieval of sequences from the genome and transcriptome sequence depositories of B. vulgaris. We thank four anonymous reviewers for insightful and very useful comments for improvement of this paper. The work was supported by the Danish Council for Independent Research, Natural Sciences [grant number 21-02-534]; and by Department of Plant and Environmental Sciences, University of Copenhagen. The funding sources were not involved in the project or the article. Publisher Copyright: © 2022 Elsevier Inc.
PY - 2022
Y1 - 2022
N2 - Barbarea, winter-cress, is a genus of 29 species in Brassicaceae, the mustard family, which has emerged as a model for evolution of plant defence and specialised metabolites. Notably, some Barbarea species have evolved the ability to produce triterpenoid saponins as the only ones in Brassicaceae, some of which make plants resistant to important herbivores. Resistance has, however, been lost in a distinct group of plants within B. vulgaris ssp. arcuata, which is genetically strongly diverged from other B. vulgaris plants. This divergence is not reflected present in taxonomy. Thus, a phylogeny is needed to understand evolution and defence in Barbarea. Here, we analysed the nuclear ITS and the plastid matK, ndhF, rps16, and psbA-trnH DNA regions from seven out of 29 Barbarea species, 57 accessions of B. vulgaris, 10 accessions of other Barbarea species, and eight outgroup species, in addition to sequences available from GenBank. All Barbarea species formed a highly supported monophyletic group, separated from sister genera. Several clades seem to have radiated within the genus with no simple branching pattern, and discordant nuclear and plastid DNA phylogenies indicate reticulate evolution and chloroplast capture. One of the complex patterns may have resulted from chloroplast capture of a non-Nordic Barbarea species not included in the study. Two pairs of species were almost identical, B. australis and B. grayi, and B. orthoceras and B. stricta. Despite hybridization, chloroplast capture, and incongruence among the plastid and nuclear DNA data, the high level of intraspecific diversity, coupled with lineage specificity, lead us to recognize three groups of Barbarea vulgaris: G-type (glabrous) and P-type (pubescent) individuals of the current B. vulgaris ssp. arcuata as two distinct groups and the current B. vulgaris ssp. vulgaris as the third. Despite the high molecular diversity below species level, the evolutionary history of the saponin-based resistance remains unsettled due to unresolved basal branching.
AB - Barbarea, winter-cress, is a genus of 29 species in Brassicaceae, the mustard family, which has emerged as a model for evolution of plant defence and specialised metabolites. Notably, some Barbarea species have evolved the ability to produce triterpenoid saponins as the only ones in Brassicaceae, some of which make plants resistant to important herbivores. Resistance has, however, been lost in a distinct group of plants within B. vulgaris ssp. arcuata, which is genetically strongly diverged from other B. vulgaris plants. This divergence is not reflected present in taxonomy. Thus, a phylogeny is needed to understand evolution and defence in Barbarea. Here, we analysed the nuclear ITS and the plastid matK, ndhF, rps16, and psbA-trnH DNA regions from seven out of 29 Barbarea species, 57 accessions of B. vulgaris, 10 accessions of other Barbarea species, and eight outgroup species, in addition to sequences available from GenBank. All Barbarea species formed a highly supported monophyletic group, separated from sister genera. Several clades seem to have radiated within the genus with no simple branching pattern, and discordant nuclear and plastid DNA phylogenies indicate reticulate evolution and chloroplast capture. One of the complex patterns may have resulted from chloroplast capture of a non-Nordic Barbarea species not included in the study. Two pairs of species were almost identical, B. australis and B. grayi, and B. orthoceras and B. stricta. Despite hybridization, chloroplast capture, and incongruence among the plastid and nuclear DNA data, the high level of intraspecific diversity, coupled with lineage specificity, lead us to recognize three groups of Barbarea vulgaris: G-type (glabrous) and P-type (pubescent) individuals of the current B. vulgaris ssp. arcuata as two distinct groups and the current B. vulgaris ssp. vulgaris as the third. Despite the high molecular diversity below species level, the evolutionary history of the saponin-based resistance remains unsettled due to unresolved basal branching.
KW - Barbarea
KW - Chloroplast capture
KW - Classification
KW - G-type and P-type
KW - Hybridization
KW - Molecular phylogeny
U2 - 10.1016/j.ympev.2022.107425
DO - 10.1016/j.ympev.2022.107425
M3 - Journal article
C2 - 35131423
AN - SCOPUS:85124514146
VL - 169
JO - Molecular Phylogenetics and Evolution
JF - Molecular Phylogenetics and Evolution
SN - 1055-7903
M1 - 107425
ER -
ID: 299750617