TY - JOUR

T1 - Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor

T2 - Effect of substrate biodegradability on conidia virulence

AU - Sala, Arnau

AU - Barrena, Raquel

AU - Meyling, Nicolai V.

AU - Artola, Adriana

PY - 2023

Y1 - 2023

N2 - using two different wastes, coupled with concentration and virulence tests of the produced conidia against thepest Tenebrio molitor. Beauveria bassiana CECT 20374 was used in solid state fermentation (SSF) operating underbatch strategy. Two substrates with different biodegradability (rice husk and beer draff) were tested, successfullyscaling from 1.5 L to 22 L bioreactors. Higher conidia production was reached using beer draff as substrate (2.5× 109 and 6.0 × 108 conidia g 1 dry matter in 1.5 and 22 L reactors respectively) highlighting air free porosityrelevance as scale-up parameter. Concentration and dose-response tests against larvae and adult Tenebrio molitorwere performed to compare strain CECT 20374 with control strain KVL 13–39 (a B. bassiana strain previouslytested against T. molitor). Virulence effect of the 22 L fermentation product of strain CECT using rice husk or beerdraff was tested against T. molitor adult stage. However, quality loses between conidia produced in agar platesand fermented products were observed (from 75 to 80% mortality in plates to 40% in rice husk and 50–60% inbeer draff fermented products respectively). The differences between plate and fermented samples also indicatedfermentation process, extraction and conservation steps as possible causes for quality losses, highlighting theneed to optimize them to maximize virulence maintenance.

AB - using two different wastes, coupled with concentration and virulence tests of the produced conidia against thepest Tenebrio molitor. Beauveria bassiana CECT 20374 was used in solid state fermentation (SSF) operating underbatch strategy. Two substrates with different biodegradability (rice husk and beer draff) were tested, successfullyscaling from 1.5 L to 22 L bioreactors. Higher conidia production was reached using beer draff as substrate (2.5× 109 and 6.0 × 108 conidia g 1 dry matter in 1.5 and 22 L reactors respectively) highlighting air free porosityrelevance as scale-up parameter. Concentration and dose-response tests against larvae and adult Tenebrio molitorwere performed to compare strain CECT 20374 with control strain KVL 13–39 (a B. bassiana strain previouslytested against T. molitor). Virulence effect of the 22 L fermentation product of strain CECT using rice husk or beerdraff was tested against T. molitor adult stage. However, quality loses between conidia produced in agar platesand fermented products were observed (from 75 to 80% mortality in plates to 40% in rice husk and 50–60% inbeer draff fermented products respectively). The differences between plate and fermented samples also indicatedfermentation process, extraction and conservation steps as possible causes for quality losses, highlighting theneed to optimize them to maximize virulence maintenance.

U2 - 10.1016/j.jenvman.2023.118059

DO - 10.1016/j.jenvman.2023.118059

M3 - Journal article

C2 - 37121182

VL - 341

JO - Journal of Environmental Management

JF - Journal of Environmental Management

SN - 0301-4797

M1 - 118059

ER -