The great divide: rhamnolipids mediate separation between P. aeruginosa and S. aureus
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The great divide : rhamnolipids mediate separation between P. aeruginosa and S. aureus. / Bru, Jean-Louis; Kasallis, Summer J; Chang, Rendell; Zhuo, Quantum; Nguyen, Jacqueline; Pham, Phillip; Warren, Elizabeth; Whiteson, Katrine; Høyland-Kroghsbo, Nina Molin; Limoli, Dominique H; Siryaporn, Albert.
In: Frontiers in Cellular and Infection Microbiology, Vol. 13, 1245874, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The great divide
T2 - rhamnolipids mediate separation between P. aeruginosa and S. aureus
AU - Bru, Jean-Louis
AU - Kasallis, Summer J
AU - Chang, Rendell
AU - Zhuo, Quantum
AU - Nguyen, Jacqueline
AU - Pham, Phillip
AU - Warren, Elizabeth
AU - Whiteson, Katrine
AU - Høyland-Kroghsbo, Nina Molin
AU - Limoli, Dominique H
AU - Siryaporn, Albert
N1 - Copyright © 2023 Bru, Kasallis, Chang, Zhuo, Nguyen, Pham, Warren, Whiteson, Høyland-Kroghsbo, Limoli and Siryaporn.
PY - 2023
Y1 - 2023
N2 - The interactions between bacterial species during infection can have significant impacts on pathogenesis. Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic bacterial pathogens that can co-infect hosts and cause serious illness. The factors that dictate whether one species outcompetes the other or whether the two species coexist are not fully understood. We investigated the role of surfactants in the interactions between these two species on a surface that enables P. aeruginosa to swarm. We found that P. aeruginosa swarms are repelled by colonies of clinical S. aureus isolates, creating physical separation between the two strains. This effect was abolished in mutants of S. aureus that were defective in the production of phenol-soluble modulins (PSMs), which form amyloid fibrils around wild-type S. aureus colonies. We investigated the mechanism that establishes physical separation between the two species using Imaging of Reflected Illuminated Structures (IRIS), which is a non-invasive imaging method that tracks the flow of surfactants produced by P. aeruginosa. We found that PSMs produced by S. aureus deflected the surfactant flow, which in turn, altered the direction of P. aeruginosa swarms. These findings show that rhamnolipids mediate physical separation between P. aeruginosa and S. aureus, which could facilitate coexistence between these species. Additionally, we found that a number of molecules repelled P. aeruginosa swarms, consistent with a surfactant deflection mechanism. These include Bacillus subtilis surfactant, the fatty acids oleic acid and linoleic acid, and the synthetic lubricant polydimethylsiloxane. Lung surfactant repelled P. aeruginosa swarms and inhibited swarm expansion altogether at higher concentration. Our results suggest that surfactant interactions could have major impacts on bacteria-bacteria and bacteria-host relationships. In addition, our findings uncover a mechanism responsible for P. aeruginosa swarm development that does not rely solely on sensing but instead is based on the flow of surfactant.
AB - The interactions between bacterial species during infection can have significant impacts on pathogenesis. Pseudomonas aeruginosa and Staphylococcus aureus are opportunistic bacterial pathogens that can co-infect hosts and cause serious illness. The factors that dictate whether one species outcompetes the other or whether the two species coexist are not fully understood. We investigated the role of surfactants in the interactions between these two species on a surface that enables P. aeruginosa to swarm. We found that P. aeruginosa swarms are repelled by colonies of clinical S. aureus isolates, creating physical separation between the two strains. This effect was abolished in mutants of S. aureus that were defective in the production of phenol-soluble modulins (PSMs), which form amyloid fibrils around wild-type S. aureus colonies. We investigated the mechanism that establishes physical separation between the two species using Imaging of Reflected Illuminated Structures (IRIS), which is a non-invasive imaging method that tracks the flow of surfactants produced by P. aeruginosa. We found that PSMs produced by S. aureus deflected the surfactant flow, which in turn, altered the direction of P. aeruginosa swarms. These findings show that rhamnolipids mediate physical separation between P. aeruginosa and S. aureus, which could facilitate coexistence between these species. Additionally, we found that a number of molecules repelled P. aeruginosa swarms, consistent with a surfactant deflection mechanism. These include Bacillus subtilis surfactant, the fatty acids oleic acid and linoleic acid, and the synthetic lubricant polydimethylsiloxane. Lung surfactant repelled P. aeruginosa swarms and inhibited swarm expansion altogether at higher concentration. Our results suggest that surfactant interactions could have major impacts on bacteria-bacteria and bacteria-host relationships. In addition, our findings uncover a mechanism responsible for P. aeruginosa swarm development that does not rely solely on sensing but instead is based on the flow of surfactant.
KW - Humans
KW - Pseudomonas aeruginosa
KW - Staphylococcus aureus/genetics
KW - Methicillin-Resistant Staphylococcus aureus
KW - Staphylococcal Infections/microbiology
KW - Biofilms
KW - Surface-Active Agents
U2 - 10.3389/fcimb.2023.1245874
DO - 10.3389/fcimb.2023.1245874
M3 - Journal article
C2 - 37780859
VL - 13
JO - Frontiers in Cellular and Infection Microbiology
JF - Frontiers in Cellular and Infection Microbiology
SN - 2235-2988
M1 - 1245874
ER -
ID: 368679045