Pruning Wound Protection Products Induce Alterations in the Wood Mycobiome Profile of Grapevines
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Pruning Wound Protection Products Induce Alterations in the Wood Mycobiome Profile of Grapevines. / Del Frari, Giovanni; Aggerbeck, Marie Rønne; Gobbi, Alex; Ingrà, Chiara; Volpi, Lorenzo; Nascimento, Teresa; Ferrandino, Alessandra; Hansen, Lars Hestbjerg; Ferreira, Ricardo Boavida.
In: Journal of Fungi, Vol. 9, No. 4, 488, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Pruning Wound Protection Products Induce Alterations in the Wood Mycobiome Profile of Grapevines
AU - Del Frari, Giovanni
AU - Aggerbeck, Marie Rønne
AU - Gobbi, Alex
AU - Ingrà, Chiara
AU - Volpi, Lorenzo
AU - Nascimento, Teresa
AU - Ferrandino, Alessandra
AU - Hansen, Lars Hestbjerg
AU - Ferreira, Ricardo Boavida
N1 - Publisher Copyright: © 2023 by the authors.
PY - 2023
Y1 - 2023
N2 - Fungal pathogens involved in grapevine trunk diseases (GTDs) may infect grapevines throughout their lifetime, from nursery to vineyard, via open wounds in stems, canes or roots. In vineyards, pruning wound protection products (PWPPs) offer the best means to reduce the chance of infection by GTD fungi. However, PWPPs may affect non-target microorganisms that comprise the natural endophytic mycobiome residing in treated canes, disrupting microbial homeostasis and indirectly influencing grapevine health. Using DNA metabarcoding, we characterized the endophytic mycobiome of one-year-old canes of cultivars Cabernet Sauvignon and Syrah in two vineyards in Portugal and Italy and assessed the impact of established and novel PWPPs on the fungal communities of treated canes. Our results reveal a large fungal diversity (176 taxa), and we report multiple genera never detected before in grapevine wood (e.g., Symmetrospora and Akenomyces). We found differences in mycobiome beta diversity when comparing vineyards (p = 0.01) but not cultivars (p > 0.05). When examining PWPP-treated canes, we detected cultivar- and vineyard-dependent alterations in both alpha and beta diversity. In addition, numerous fungal taxa were over- or under-represented when compared to control canes. Among them, Epicoccum sp., a beneficial genus with biological control potential, was negatively affected by selected PWPPs. This study demonstrates that PWPPs induce alterations in the fungal communities of grapevines, requiring an urgent evaluation of their direct and indirect effects on plants health with consideration of factors such as climatic conditions and yearly variations, in order to better advise viticulturists and policy makers.
AB - Fungal pathogens involved in grapevine trunk diseases (GTDs) may infect grapevines throughout their lifetime, from nursery to vineyard, via open wounds in stems, canes or roots. In vineyards, pruning wound protection products (PWPPs) offer the best means to reduce the chance of infection by GTD fungi. However, PWPPs may affect non-target microorganisms that comprise the natural endophytic mycobiome residing in treated canes, disrupting microbial homeostasis and indirectly influencing grapevine health. Using DNA metabarcoding, we characterized the endophytic mycobiome of one-year-old canes of cultivars Cabernet Sauvignon and Syrah in two vineyards in Portugal and Italy and assessed the impact of established and novel PWPPs on the fungal communities of treated canes. Our results reveal a large fungal diversity (176 taxa), and we report multiple genera never detected before in grapevine wood (e.g., Symmetrospora and Akenomyces). We found differences in mycobiome beta diversity when comparing vineyards (p = 0.01) but not cultivars (p > 0.05). When examining PWPP-treated canes, we detected cultivar- and vineyard-dependent alterations in both alpha and beta diversity. In addition, numerous fungal taxa were over- or under-represented when compared to control canes. Among them, Epicoccum sp., a beneficial genus with biological control potential, was negatively affected by selected PWPPs. This study demonstrates that PWPPs induce alterations in the fungal communities of grapevines, requiring an urgent evaluation of their direct and indirect effects on plants health with consideration of factors such as climatic conditions and yearly variations, in order to better advise viticulturists and policy makers.
KW - biological control
KW - Cabernet Sauvignon
KW - copper
KW - fungicides
KW - grapevine trunk diseases
KW - microbiome
KW - Syrah
KW - Trichoderma
KW - Vitis vinifera
U2 - 10.3390/jof9040488
DO - 10.3390/jof9040488
M3 - Journal article
C2 - 37108942
AN - SCOPUS:85154023293
VL - 9
JO - Journal of Fungi
JF - Journal of Fungi
SN - 2309-608X
IS - 4
M1 - 488
ER -
ID: 347977158