Constructing and screening a metagenomic library of a cold and alkaline extreme environment
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Natural cold or alkaline environments are common on Earth. A rare combination of these two extremes is found in the permanently cold (less than 6 °C) and alkaline (pH above 10) ikaite columns in the Ikka Fjord in Southern Greenland. Bioprospecting efforts have established the ikaite columns as a source of bacteria and enzymes adapted to these conditions. They have also highlighted the limitations of cultivation-based methods in this extreme environment and metagenomic approaches may provide access to novel extremophilic enzymes from the uncultured majority of bacteria. Here, we describe the construction and screening of a metagenomic library of the prokaryotic community inhabiting the ikaite columns.
Original language | English |
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Title of host publication | Metagenomics |
Editors | Wolfgang R. Streit, Rolf Daniel |
Number of pages | 13 |
Publisher | Springer |
Publication date | 2017 |
Pages | 43-55 |
Chapter | 4 |
ISBN (Print) | 978-1-4939-6689-9 |
ISBN (Electronic) | 978-1-4939-6691-2 |
DOIs | |
Publication status | Published - 2017 |
Series | Methods in Molecular Biology |
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Volume | 1539 |
ISSN | 1064-3745 |
- Journal Article
Research areas
ID: 180819418