The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism. / Song, Yu; Li, Gang; Nowak, Jacqueline; Zhang, Xiaoqing; Xu, Dongbei; Yang, Xiujuan; Huang, Guoqiang; Liang, Wanqi; Yang, Litao; Wang, Canhua; Bulone, Vincent; Nikoloski, Zoran; Hu, Jianping; Persson, Staffan; Zhang, Dabing.
In: Plant Physiology, Vol. 181, No. 2, 2019, p. 630-644.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism
AU - Song, Yu
AU - Li, Gang
AU - Nowak, Jacqueline
AU - Zhang, Xiaoqing
AU - Xu, Dongbei
AU - Yang, Xiujuan
AU - Huang, Guoqiang
AU - Liang, Wanqi
AU - Yang, Litao
AU - Wang, Canhua
AU - Bulone, Vincent
AU - Nikoloski, Zoran
AU - Hu, Jianping
AU - Persson, Staffan
AU - Zhang, Dabing
PY - 2019
Y1 - 2019
N2 - Light and gravity are two key determinants in orientating plant stems for proper growth and development. The organization and dynamics of the actin cytoskeleton are essential for cell biology and critically regulated by actin-binding proteins. However, the role of actin cytoskeleton in shoot negative gravitropism remains controversial. In this work, we report that the actin-binding protein Rice Morphology Determinant (RMD) promotes reorganization of the actin cytoskeleton in rice (Oryza sativa) shoots. The changes in actin organization are associated with the ability of the rice shoots to respond to negative gravitropism. Here, light-grown rmd mutant shoots exhibited agravitropic phenotypes. By contrast, etiolated rmd shoots displayed normal negative shoot gravitropism. Furthermore, we show that RMD maintains an actin configuration that promotes statolith mobility in gravisensing endodermal cells, and for proper auxin distribution in light-grown, but not dark-grown, shoots. RMD gene expression is diurnally controlled and directly repressed by the phytochrome-interacting factor-like protein OsPIL16. Consequently, overexpression of OsPIL16 led to gravisensing and actin patterning defects that phenocopied the rmd mutant. Our findings outline a mechanism that links light signaling and gravity perception for straight shoot growth in rice.
AB - Light and gravity are two key determinants in orientating plant stems for proper growth and development. The organization and dynamics of the actin cytoskeleton are essential for cell biology and critically regulated by actin-binding proteins. However, the role of actin cytoskeleton in shoot negative gravitropism remains controversial. In this work, we report that the actin-binding protein Rice Morphology Determinant (RMD) promotes reorganization of the actin cytoskeleton in rice (Oryza sativa) shoots. The changes in actin organization are associated with the ability of the rice shoots to respond to negative gravitropism. Here, light-grown rmd mutant shoots exhibited agravitropic phenotypes. By contrast, etiolated rmd shoots displayed normal negative shoot gravitropism. Furthermore, we show that RMD maintains an actin configuration that promotes statolith mobility in gravisensing endodermal cells, and for proper auxin distribution in light-grown, but not dark-grown, shoots. RMD gene expression is diurnally controlled and directly repressed by the phytochrome-interacting factor-like protein OsPIL16. Consequently, overexpression of OsPIL16 led to gravisensing and actin patterning defects that phenocopied the rmd mutant. Our findings outline a mechanism that links light signaling and gravity perception for straight shoot growth in rice.
U2 - 10.1104/pp.19.00497
DO - 10.1104/pp.19.00497
M3 - Journal article
C2 - 31416828
VL - 181
SP - 630
EP - 644
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 2
ER -
ID: 247692572