Bright fluorescent vacuolar marker lines allow vacuolar tracing across multiple tissues and stress conditions in rice

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Bright fluorescent vacuolar marker lines allow vacuolar tracing across multiple tissues and stress conditions in rice. / Cao, Yiran; Cai, Wenguo; Chen, Xiaofei; Chen, Mingjiao; Chu, Jianjun; Liang, Wanqi; Persson, Staffan; Liu, Zengyu; Zhang, Dabing.

In: International Journal of Molecular Sciences, Vol. 21, No. 12, 4203, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cao, Y, Cai, W, Chen, X, Chen, M, Chu, J, Liang, W, Persson, S, Liu, Z & Zhang, D 2020, 'Bright fluorescent vacuolar marker lines allow vacuolar tracing across multiple tissues and stress conditions in rice', International Journal of Molecular Sciences, vol. 21, no. 12, 4203. https://doi.org/10.3390/ijms21124203

APA

Cao, Y., Cai, W., Chen, X., Chen, M., Chu, J., Liang, W., Persson, S., Liu, Z., & Zhang, D. (2020). Bright fluorescent vacuolar marker lines allow vacuolar tracing across multiple tissues and stress conditions in rice. International Journal of Molecular Sciences, 21(12), [4203]. https://doi.org/10.3390/ijms21124203

Vancouver

Cao Y, Cai W, Chen X, Chen M, Chu J, Liang W et al. Bright fluorescent vacuolar marker lines allow vacuolar tracing across multiple tissues and stress conditions in rice. International Journal of Molecular Sciences. 2020;21(12). 4203. https://doi.org/10.3390/ijms21124203

Author

Cao, Yiran ; Cai, Wenguo ; Chen, Xiaofei ; Chen, Mingjiao ; Chu, Jianjun ; Liang, Wanqi ; Persson, Staffan ; Liu, Zengyu ; Zhang, Dabing. / Bright fluorescent vacuolar marker lines allow vacuolar tracing across multiple tissues and stress conditions in rice. In: International Journal of Molecular Sciences. 2020 ; Vol. 21, No. 12.

Bibtex

@article{d69da27c393b4448a3171ad1c40ce200,
title = "Bright fluorescent vacuolar marker lines allow vacuolar tracing across multiple tissues and stress conditions in rice",
abstract = "The vacuole is indispensable for cells to maintain their water potential and to respond to environmental changes. Nevertheless, investigations of vacuole morphology and its functions have been limited to Arabidopsis thaliana with few studies in the model crop rice (Oryza sativa). Here, we report the establishment of bright rice vacuole fluorescent reporter systems using OsTIP1;1, a tonoplast water channel protein, fused to either an enhanced green fluorescent protein or an mCherry red fluorescent protein. We used the corresponding transgenic rice lines to trace the vacuole morphology in roots, leaves, anthers, and pollen grains. Notably, we observed dynamic changes in vacuole morphologies in pollen and root epidermis that corresponded to their developmental states as well as vacuole shape alterations in response to abiotic stresses. Our results indicate that the application of our vacuole markers may aid in understanding rice vacuole function and structure across different tissues and environmental conditions in rice.",
keywords = "Fluorescent marker, Oryza sativa, Tonoplast intrinsic protein, Vacuole",
author = "Yiran Cao and Wenguo Cai and Xiaofei Chen and Mingjiao Chen and Jianjun Chu and Wanqi Liang and Staffan Persson and Zengyu Liu and Dabing Zhang",
year = "2020",
doi = "10.3390/ijms21124203",
language = "English",
volume = "21",
journal = "International Journal of Molecular Sciences (CD-ROM)",
issn = "1424-6783",
publisher = "M D P I AG",
number = "12",

}

RIS

TY - JOUR

T1 - Bright fluorescent vacuolar marker lines allow vacuolar tracing across multiple tissues and stress conditions in rice

AU - Cao, Yiran

AU - Cai, Wenguo

AU - Chen, Xiaofei

AU - Chen, Mingjiao

AU - Chu, Jianjun

AU - Liang, Wanqi

AU - Persson, Staffan

AU - Liu, Zengyu

AU - Zhang, Dabing

PY - 2020

Y1 - 2020

N2 - The vacuole is indispensable for cells to maintain their water potential and to respond to environmental changes. Nevertheless, investigations of vacuole morphology and its functions have been limited to Arabidopsis thaliana with few studies in the model crop rice (Oryza sativa). Here, we report the establishment of bright rice vacuole fluorescent reporter systems using OsTIP1;1, a tonoplast water channel protein, fused to either an enhanced green fluorescent protein or an mCherry red fluorescent protein. We used the corresponding transgenic rice lines to trace the vacuole morphology in roots, leaves, anthers, and pollen grains. Notably, we observed dynamic changes in vacuole morphologies in pollen and root epidermis that corresponded to their developmental states as well as vacuole shape alterations in response to abiotic stresses. Our results indicate that the application of our vacuole markers may aid in understanding rice vacuole function and structure across different tissues and environmental conditions in rice.

AB - The vacuole is indispensable for cells to maintain their water potential and to respond to environmental changes. Nevertheless, investigations of vacuole morphology and its functions have been limited to Arabidopsis thaliana with few studies in the model crop rice (Oryza sativa). Here, we report the establishment of bright rice vacuole fluorescent reporter systems using OsTIP1;1, a tonoplast water channel protein, fused to either an enhanced green fluorescent protein or an mCherry red fluorescent protein. We used the corresponding transgenic rice lines to trace the vacuole morphology in roots, leaves, anthers, and pollen grains. Notably, we observed dynamic changes in vacuole morphologies in pollen and root epidermis that corresponded to their developmental states as well as vacuole shape alterations in response to abiotic stresses. Our results indicate that the application of our vacuole markers may aid in understanding rice vacuole function and structure across different tissues and environmental conditions in rice.

KW - Fluorescent marker

KW - Oryza sativa

KW - Tonoplast intrinsic protein

KW - Vacuole

U2 - 10.3390/ijms21124203

DO - 10.3390/ijms21124203

M3 - Journal article

C2 - 32545623

AN - SCOPUS:85086369474

VL - 21

JO - International Journal of Molecular Sciences (CD-ROM)

JF - International Journal of Molecular Sciences (CD-ROM)

SN - 1424-6783

IS - 12

M1 - 4203

ER -

ID: 260353911