Protocol for analyzing root halotropism using split-agar system in Arabidopsis thaliana

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Plant roots sense salt gradients in soil to avoid saline environments through halotropism. Here, we present a protocol to study halotropism with an optimized split-agar system that simulates the salt gradient in soil. We describe steps for preparation of the split-agar system, measurement of Na+, and observation of root bending. We then detail segmentation of root cells and visualization of microtubules and cellulose synthases. This system is simple to operate and has broader applications, such as hydrotropism and chemotropism. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022).1

OriginalsprogEngelsk
Artikelnummer102157
TidsskriftSTAR Protocols
Vol/bind4
Udgave nummer2
Antal sider16
ISSN2666-1667
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This work was supported by the National Natural Science Foundation of China (NSFC grant no. 31970293 to Y.Z. and 32100237 to B.Y.), the Strategic Priority Research Program of the Chinese Academy of Sciences (grant no. XDB27040107 ), and the Science and Tec Technology Commission of Shanghai Municipality ( 22ZR1481400 ). S.P. acknowledges the financial aid of an ARC Discovery grant ( DP19001941 ) and Villum Investigator (Project ID: 25915 ), DNRF Chair ( DNRF155 ), Novo Nordisk Laureate ( NNF19OC0056076 ), Novo Nordisk Emerging Investigator ( NNF20OC0060564 ), and Lundbeck Foundation (experiment grant, R346-2020-1546 ) grants.

Publisher Copyright:
© 2023 The Author(s)

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