Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Extracellular acidification or alkalization is a common response to many plant-signaling peptides and microbial elicitors. This may be a result of peptide-mediated regulation of plasma membrane-localized ion transporters, such as the plasma membrane H+-ATPase. Early responses to some signaling peptides can therefore be analyzed by assaying H+-pumping across the plasma membrane. We describe a set-up suited for the purification of plasma membranes by aqueous two-phase partitioning from a small sample of Arabidopsis seedlings. Seedlings are grown in a liquid culture, suited for the analysis of in vivo peptide treatment. Additionally, we describe how to measure the H+-pumping activity of the plasma membrane H+-ATPase using the fluorescent probe ACMA.

TitelPlant Peptide Hormones and Growth Factors
Antal sider13
ForlagHumana Press
ISBN (Trykt)978-1-0716-3510-0
ISBN (Elektronisk)978-1-0716-3511-7
StatusUdgivet - 2024
NavnMethods in Molecular Biology

Bibliografisk note

Publisher Copyright:
© 2024, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

ID: 389549508