Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings

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Standard

Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings. / Havshøi, Nanna Weise; Fuglsang, Anja Thoe.

Plant Peptide Hormones and Growth Factors. Humana Press, 2024. s. 91-103 (Methods in Molecular Biology, Bind 2731).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Havshøi, NW & Fuglsang, AT 2024, Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings. i Plant Peptide Hormones and Growth Factors. Humana Press, Methods in Molecular Biology, bind 2731, s. 91-103. https://doi.org/10.1007/978-1-0716-3511-7_7

APA

Havshøi, N. W., & Fuglsang, A. T. (2024). Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings. I Plant Peptide Hormones and Growth Factors (s. 91-103). Humana Press. Methods in Molecular Biology Bind 2731 https://doi.org/10.1007/978-1-0716-3511-7_7

Vancouver

Havshøi NW, Fuglsang AT. Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings. I Plant Peptide Hormones and Growth Factors. Humana Press. 2024. s. 91-103. (Methods in Molecular Biology, Bind 2731). https://doi.org/10.1007/978-1-0716-3511-7_7

Author

Havshøi, Nanna Weise ; Fuglsang, Anja Thoe. / Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings. Plant Peptide Hormones and Growth Factors. Humana Press, 2024. s. 91-103 (Methods in Molecular Biology, Bind 2731).

Bibtex

@inbook{04543a660b524153a72b2ec2fd787382,
title = "Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings",
abstract = "Extracellular acidification or alkalization is a common response to many plant-signaling peptides and microbial elicitors. This may be a result of peptide-mediated regulation of plasma membrane-localized ion transporters, such as the plasma membrane H+-ATPase. Early responses to some signaling peptides can therefore be analyzed by assaying H+-pumping across the plasma membrane. We describe a set-up suited for the purification of plasma membranes by aqueous two-phase partitioning from a small sample of Arabidopsis seedlings. Seedlings are grown in a liquid culture, suited for the analysis of in vivo peptide treatment. Additionally, we describe how to measure the H+-pumping activity of the plasma membrane H+-ATPase using the fluorescent probe ACMA.",
keywords = "H pumping assay, H-ATPase, In vivo peptide treatment, Liquid seedling culture, Membrane vesicles, Plasma membrane, Two-phase partitioning",
author = "Havsh{\o}i, {Nanna Weise} and Fuglsang, {Anja Thoe}",
note = "Publisher Copyright: {\textcopyright} 2024, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2024",
doi = "10.1007/978-1-0716-3511-7_7",
language = "English",
isbn = "978-1-0716-3510-0",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "91--103",
booktitle = "Plant Peptide Hormones and Growth Factors",
address = "United States",

}

RIS

TY - CHAP

T1 - Assaying the Effect of Peptide Treatment on H+-Pumping Activity in Plasma Membranes from Arabidopsis Seedlings

AU - Havshøi, Nanna Weise

AU - Fuglsang, Anja Thoe

N1 - Publisher Copyright: © 2024, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2024

Y1 - 2024

N2 - Extracellular acidification or alkalization is a common response to many plant-signaling peptides and microbial elicitors. This may be a result of peptide-mediated regulation of plasma membrane-localized ion transporters, such as the plasma membrane H+-ATPase. Early responses to some signaling peptides can therefore be analyzed by assaying H+-pumping across the plasma membrane. We describe a set-up suited for the purification of plasma membranes by aqueous two-phase partitioning from a small sample of Arabidopsis seedlings. Seedlings are grown in a liquid culture, suited for the analysis of in vivo peptide treatment. Additionally, we describe how to measure the H+-pumping activity of the plasma membrane H+-ATPase using the fluorescent probe ACMA.

AB - Extracellular acidification or alkalization is a common response to many plant-signaling peptides and microbial elicitors. This may be a result of peptide-mediated regulation of plasma membrane-localized ion transporters, such as the plasma membrane H+-ATPase. Early responses to some signaling peptides can therefore be analyzed by assaying H+-pumping across the plasma membrane. We describe a set-up suited for the purification of plasma membranes by aqueous two-phase partitioning from a small sample of Arabidopsis seedlings. Seedlings are grown in a liquid culture, suited for the analysis of in vivo peptide treatment. Additionally, we describe how to measure the H+-pumping activity of the plasma membrane H+-ATPase using the fluorescent probe ACMA.

KW - H pumping assay

KW - H-ATPase

KW - In vivo peptide treatment

KW - Liquid seedling culture

KW - Membrane vesicles

KW - Plasma membrane

KW - Two-phase partitioning

U2 - 10.1007/978-1-0716-3511-7_7

DO - 10.1007/978-1-0716-3511-7_7

M3 - Book chapter

C2 - 38019428

AN - SCOPUS:85178498412

SN - 978-1-0716-3510-0

T3 - Methods in Molecular Biology

SP - 91

EP - 103

BT - Plant Peptide Hormones and Growth Factors

PB - Humana Press

ER -

ID: 389549508