Actomyosin and CSI1/POM2 cooperate to deliver cellulose synthase from Golgi to cortical microtubules in Arabidopsis

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  • Lu Liu
  • Ting Wang
  • Yifan Bai
  • Pengcheng Yan
  • Liufeng Dai
  • Pingzhou Du
  • Persson, Staffan
  • Yi Zhang

As one of the major components of plant cell walls, cellulose is crucial for plant growth and development. Cellulose is synthesized by cellulose synthase (CesA) complexes (CSCs), which are trafficked and delivered from the Golgi apparatus to the plasma membrane. How CesAs are released from Golgi remains largely unclear. In this study, we observed that STELLO (STL) family proteins localized at a group of small CesA-containing compartments called Small CesA compartments (SmaCCs) or microtubule-associated CesA compartments (MASCs). The STL-labeled SmaCCs/MASCs were directly derived from Golgi through a membrane-stretching process: membrane-patches of Golgi attached to cortical microtubules, which led to emergence of membrane-tails that finally ruptured to generate SmaCCs/MASCs associated with the cortical microtubules. While myosin propelled the movement of Golgi along actin filaments to stretch the tails, the CesA-microtubule linker protein, CSI1/POM2 was indispensable for the tight anchor of the membrane-tail ends at cortical microtubules. Together, our data reveal a non-canonical delivery route to the plasma membrane of a major enzyme complex in plant biology.

OriginalsprogEngelsk
Artikelnummer7442
TidsskriftNature Communications
Vol/bind14
Antal sider13
ISSN2041-1723
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
We acknowledge the helpful discussion with Professor Paul Dupree (Cambridge University) at the initial stages of the project. We acknowledge the experimental technology center for life sciences, Beijing Normal University, and thank Dr. Xiaoyan Zhang for technical support. We acknowledge the assistance of Imaging Core Facility of Protein Research Center for Technology Development for assistance of using the high-speed laser confocal living cell workstation. This work was supported by grants from the National Natural Science Foundation of China (32070194, 32270350 and 31870174 to Y.Z., and 32100279 to T.W.). S.P. acknowledges the financial aid of Villum Investigator (Project ID: 25915), DNRF Chair (DNRF155) and Novo Nordisk Laureate (NNF19OC0056076), Novo Nordisk Emerging Investigator (NNF20OC0060564); Novo Nordisk Data Science (NNF0068884) and Lundbeck foundation (Experiment grant, R346-2020-1546) grants.

Funding Information:
We acknowledge the helpful discussion with Professor Paul Dupree (Cambridge University) at the initial stages of the project. We acknowledge the experimental technology center for life sciences, Beijing Normal University, and thank Dr. Xiaoyan Zhang for technical support. We acknowledge the assistance of Imaging Core Facility of Protein Research Center for Technology Development for assistance of using the high-speed laser confocal living cell workstation. This work was supported by grants from the National Natural Science Foundation of China (32070194, 32270350 and 31870174 to Y.Z., and 32100279 to T.W.). S.P. acknowledges the financial aid of Villum Investigator (Project ID: 25915), DNRF Chair (DNRF155) and Novo Nordisk Laureate (NNF19OC0056076), Novo Nordisk Emerging Investigator (NNF20OC0060564); Novo Nordisk Data Science (NNF0068884) and Lundbeck foundation (Experiment grant, R346-2020-1546) grants.

Publisher Copyright:
© 2023, The Author(s).

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