A receptor-like kinase controls the amplitude of secondary cell wall synthesis in rice

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

  • Wenguo Cai
  • Jun Hong
  • Zengyu Liu
  • Wenyu Wang
  • Jiao Zhang
  • Gynheung An
  • Wanqi Liang
  • Persson, Staffan
  • Dabing Zhang

Cell wall expansion is a key element in determining plant morphology and growth, and cell wall integrity changes are relayed to the cell to fine-tune growth responses. Here, we show that variations in the ectodomain of a cell wall-associated receptor-like kinase, WAK10, in temperate Oryza japonica accessions differentially amplify fluctuations in cell wall integrity to control rice stem height. Mutation in the WAK10 gene exhibited increased cell wall thickening in stem sclerenchyma and reduced cell expansion in the stem. Two WAK10 ectodomain variants bound pectic oligosaccharides with different affinities. The pectic oligosaccharide binding regulated WAK10 phosphorylation activity, the amplitude of secondary wall deposition, and ultimately, stem height. Rice population analyses revealed active enrichment of the short-stem WAK10 ectodomain alleles in japonica subspecies during domestication. Our study outlines not only a mechanism for how variations in ligand affinities of a receptor kinase control cell wall biosynthesis and plant growth, but it also provides breeding targets for new semi-dwarf rice cultivars.

OriginalsprogEngelsk
TidsskriftCurrent Biology
Vol/bind33
Udgave nummer3
Sider (fra-til)498-506.e6
ISSN0960-9822
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
We thank Wensheng Wang and Zhikang Li (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China) for the O. sativa accessions, Lizhi Gao (Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.) for providing O. rufipogon accessions, Mingjiao Chen and Xiaofei Chen (Shanghai Jiao Tong University) for managing the plant materials and rice genetic transformation, and Natalie Betts for editing this manuscript. This work was financially supported by the National Natural Science Foundation of China (31970803 and 31861163002), Yazhou Bay Seed Laboratory Project (B21HJ8104), Jiangsu Provincial Key R&D Programme (BE2021323), Australian Research Council Discovery Project grants (DP210100956 and DP230102476), Australian Research Council Training Centre for Accelerated Future Crop Development (IC210100047), and Australian Research Council Linkage (LP210301062) to D.Z.; Postdoctoral Science Foundation (2017M621451) to W.C.; and Villum, two Novo Nordisk Foundation, Danish National Research Foundation, and Australian Research Council Discovery Project grants (25915, 19OC0056076, 20OC0060564, DNRF155, and DP190101941, respectively) to S.P. D.Z. and J.H. initiated the project. W.C. and D.Z. conceived the project and designed the experiments. W.C. carried out most of the molecular and regulatory experiments. J.H. did the GWAS and in silico analysis. Z.L. carried out the microscopy imaging. W.W. J.Z. and G.A. conducted plant material preparation. W.C. J.H. Z.L. G.A. W.L. S.P. and D.Z. analyzed the results and discussion. W.C. S.P. and D.Z. wrote the manuscript with input from all authors. The authors declare no competing interests.

Funding Information:
We thank Wensheng Wang and Zhikang Li (Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China) for the O. sativa accessions, Lizhi Gao (Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.) for providing O. rufipogon accessions, Mingjiao Chen and Xiaofei Chen (Shanghai Jiao Tong University) for managing the plant materials and rice genetic transformation, and Natalie Betts for editing this manuscript. This work was financially supported by the National Natural Science Foundation of China ( 31970803 and 31861163002 ), Yazhou Bay Seed Laboratory Project ( B21HJ8104 ), Jiangsu Provincial Key R&D Programme ( BE2021323 ), Australian Research Council Discovery Project grants ( DP210100956 and DP230102476 ), Australian Research Council Training Centre for Accelerated Future Crop Development ( IC210100047 ), and Australian Research Council Linkage ( LP210301062 ) to D.Z.; Postdoctoral Science Foundation ( 2017M621451 ) to W.C.; and Villum , two Novo Nordisk Foundation , Danish National Research Foundation , and Australian Research Council Discovery Project grants ( 25915 , 19OC0056076 , 20OC0060564 , DNRF155 , and DP190101941 , respectively) to S.P.

Publisher Copyright:
© 2022

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