Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi
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Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi. / Keyes, Sam; van Veelen, Arjen; McKay Fletcher, Dan; Scotson, Callum; Koebernick, Nico; Petroselli, Chiara; Williams, Katherine; Ruiz, Siul; Cooper, Laura; Mayon, Robbie; Duncan, Simon; Dumont, Marc; Jakobsen, Iver; Oldroyd, Giles; Tkacz, Andrzej; Poole, Philip; Mosselmans, Fred; Borca, Camelia; Huthwelker, Thomas; Jones, David L.; Roose, Tiina.
In: New Phytologist, Vol. 234, No. 2, 2022, p. 688-703.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi
AU - Keyes, Sam
AU - van Veelen, Arjen
AU - McKay Fletcher, Dan
AU - Scotson, Callum
AU - Koebernick, Nico
AU - Petroselli, Chiara
AU - Williams, Katherine
AU - Ruiz, Siul
AU - Cooper, Laura
AU - Mayon, Robbie
AU - Duncan, Simon
AU - Dumont, Marc
AU - Jakobsen, Iver
AU - Oldroyd, Giles
AU - Tkacz, Andrzej
AU - Poole, Philip
AU - Mosselmans, Fred
AU - Borca, Camelia
AU - Huthwelker, Thomas
AU - Jones, David L.
AU - Roose, Tiina
N1 - Publisher Copyright: © 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation
PY - 2022
Y1 - 2022
N2 - Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated. We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling. We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co-locate with areas of high P and low Al, and preferentially associate with organic-type P species over Al-rich inorganic P. We discovered that AMF avoid Al-rich areas as a source of P. Sulphur-rich regions were found to be correlated with higher hyphal density and an increased organic-associated P-pool, whilst oxidized S-species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome-related. Our experimentally-validated model led to an estimate of P-uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated – a result with significant implications for the modelling of plant–soil–AMF interactions.
AB - Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated. We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling. We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co-locate with areas of high P and low Al, and preferentially associate with organic-type P species over Al-rich inorganic P. We discovered that AMF avoid Al-rich areas as a source of P. Sulphur-rich regions were found to be correlated with higher hyphal density and an increased organic-associated P-pool, whilst oxidized S-species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome-related. Our experimentally-validated model led to an estimate of P-uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated – a result with significant implications for the modelling of plant–soil–AMF interactions.
KW - mycorrhizas
KW - plant phosphorus uptake
KW - rhizosphere modelling
KW - synchrotron
KW - X-ray computed tomography
KW - X-ray fluorescence
U2 - 10.1111/nph.17980
DO - 10.1111/nph.17980
M3 - Journal article
C2 - 35043984
AN - SCOPUS:85124601328
VL - 234
SP - 688
EP - 703
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 2
ER -
ID: 298478832