Impact of Host Plant Species and Whitefly Species on Feeding Behavior of Bemisia tabaci
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Impact of Host Plant Species and Whitefly Species on Feeding Behavior of Bemisia tabaci. / Milenovic, Milan; Wosula, Everlyne Nafula; Rapisarda, Carmelo; Legg, James Peter.
In: Frontiers in Plant Science, Vol. 10, 1, 01.2019, p. 1-14.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Impact of Host Plant Species and Whitefly Species on Feeding Behavior of Bemisia tabaci
AU - Milenovic, Milan
AU - Wosula, Everlyne Nafula
AU - Rapisarda, Carmelo
AU - Legg, James Peter
PY - 2019/1
Y1 - 2019/1
N2 - Whiteflies of the Bemisia tabaci species complex are economically important pests of cassava. In Africa, they cause greatest damage through vectoring viruses responsible for cassava mosaic disease and cassava brown streak disease. Several cryptic species from the B. tabaci complex colonize cassava and neighboring crops, but the feeding interactions between the different crops and B. tabaci species are unknown. The electrical penetration graph (EPG) technique makes it possible to conduct detailed feeding studies of sap-sucking insects by creating an electric circuit through the insect and the plant. The apparatus measures the voltage fluctuations while the wired-up insect feeds and produces graphs that describe feeding behavior. We utilized EPG to explore the feeding behavior of cassava-colonizing whiteflies (SSA1-SG3) on cassava, sweet potato, tomato, and cotton; and sweet potato-colonizing whiteflies (MED and IO) on cassava and sweet potato. Results show that: (1) feeding of SSA1-SG3 is not restricted to cassava. The least preferred host for SSA1-SG3 was tomato, where probing was delayed by 99 min compared to 10 min on other hosts, furthermore mean duration of phloem ingestion events was 36 min compared to 260 min on cassava. (2) Feeding of MED on cassava appeared to be non-functional, as it was characterized by short total phloem ingestion periods (<1 h) and few, short ingestion events, in contrast to feeding on sweet potato which was characterized by long phloem ingestion periods (>5 h). (3) Wire diameter affects the feeding in a statistically and practically significant manner. Implications for whitefly control and studies of host whitefly resistance are discussed.
AB - Whiteflies of the Bemisia tabaci species complex are economically important pests of cassava. In Africa, they cause greatest damage through vectoring viruses responsible for cassava mosaic disease and cassava brown streak disease. Several cryptic species from the B. tabaci complex colonize cassava and neighboring crops, but the feeding interactions between the different crops and B. tabaci species are unknown. The electrical penetration graph (EPG) technique makes it possible to conduct detailed feeding studies of sap-sucking insects by creating an electric circuit through the insect and the plant. The apparatus measures the voltage fluctuations while the wired-up insect feeds and produces graphs that describe feeding behavior. We utilized EPG to explore the feeding behavior of cassava-colonizing whiteflies (SSA1-SG3) on cassava, sweet potato, tomato, and cotton; and sweet potato-colonizing whiteflies (MED and IO) on cassava and sweet potato. Results show that: (1) feeding of SSA1-SG3 is not restricted to cassava. The least preferred host for SSA1-SG3 was tomato, where probing was delayed by 99 min compared to 10 min on other hosts, furthermore mean duration of phloem ingestion events was 36 min compared to 260 min on cassava. (2) Feeding of MED on cassava appeared to be non-functional, as it was characterized by short total phloem ingestion periods (<1 h) and few, short ingestion events, in contrast to feeding on sweet potato which was characterized by long phloem ingestion periods (>5 h). (3) Wire diameter affects the feeding in a statistically and practically significant manner. Implications for whitefly control and studies of host whitefly resistance are discussed.
KW - Bemisia tabaci
KW - Cassava
KW - EPG
KW - Feeding behavior
KW - Sweet potato
KW - Whitefly
U2 - 10.3389/fpls.2019.00001
DO - 10.3389/fpls.2019.00001
M3 - Journal article
C2 - 30723482
AN - SCOPUS:85061845273
VL - 10
SP - 1
EP - 14
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 1
ER -
ID: 222807752