TY - JOUR

T1 - Travel light

T2 - Essential packing for membrane proteins with an active lifestyle

AU - Johansen, Nicolai Tidemand

AU - Tidemand, Frederik Grønbæk

AU - Pedersen, Martin Cramer

AU - Arleth, Lise

N1 - Publisher Copyright: © 2022 The Authors

PY - 2023

Y1 - 2023

N2 - We review the considerable progress during the recent decade in the endeavours of designing, optimising, and utilising carrier particle systems for structural and functional studies of membrane proteins in near-native environments. New and improved systems are constantly emerging, novel studies push the perceived limits of a given carrier system, and specific carrier systems consolidate and entrench themselves as the system of choice for particular classes of target membrane protein systems. This review covers the most frequently used carrier systems for such studies and emphasises similarities and differences between these systems as well as current trends and future directions for the field. Particular interest is devoted to the biophysical properties and membrane mimicking ability of each system and the manner in which this may impact an embedded membrane protein and an eventual structural or functional study.

AB - We review the considerable progress during the recent decade in the endeavours of designing, optimising, and utilising carrier particle systems for structural and functional studies of membrane proteins in near-native environments. New and improved systems are constantly emerging, novel studies push the perceived limits of a given carrier system, and specific carrier systems consolidate and entrench themselves as the system of choice for particular classes of target membrane protein systems. This review covers the most frequently used carrier systems for such studies and emphasises similarities and differences between these systems as well as current trends and future directions for the field. Particular interest is devoted to the biophysical properties and membrane mimicking ability of each system and the manner in which this may impact an embedded membrane protein and an eventual structural or functional study.

KW - Amphiphilic polymers

KW - Carrier system

KW - Membrane protein

KW - Micelle

KW - Nanodisc

U2 - 10.1016/j.biochi.2022.07.014

DO - 10.1016/j.biochi.2022.07.014

M3 - Review

C2 - 35963461

AN - SCOPUS:85136266700

VL - 205

SP - 3

EP - 26

JO - Biochimie

JF - Biochimie

SN - 0300-9084

ER -