The Mobilome: Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements
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The Mobilome : Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements. / Browne, Patrick Denis; Kot, Witold; Jørgensen, Tue Sparholt; Hansen, Lars Hestbjerg.
Horizontal Gene Transfer: Methods and Protocols. red. / Fernando de la Cruz. Humana Press, 2020. s. 253-264 (Methods in Molecular Biology, Bind 2075).Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - The Mobilome
T2 - Metagenomic Analysis of Circular Plasmids, Viruses, and Other Extrachromosomal Elements
AU - Browne, Patrick Denis
AU - Kot, Witold
AU - Jørgensen, Tue Sparholt
AU - Hansen, Lars Hestbjerg
PY - 2020
Y1 - 2020
N2 - Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.
AB - Isolation, sequencing, and analysis of circular genetic elements bring new insights to mobile genetic elements related to microbial ecology. One method used to study circular plasmids, viruses, and other elements is called the mobilome method. The mobilome method presented here is an unamplified mobilome approach allowing fast isolation of circular DNA elements from a variety of samples followed by directly building unamplified Illumina-compatible sequencing libraries using enzymatic tagging and fragmentation. Several methods for bioinformatic analysis of mobilome data are also suggested.
KW - Circular DNA elements
KW - Horizontal gene transfer
KW - Metagenomics
KW - MGE
KW - Mobile genetic elements
KW - Mobilome
KW - Plasmid
KW - Plasmidome
U2 - 10.1007/978-1-4939-9877-7_18
DO - 10.1007/978-1-4939-9877-7_18
M3 - Book chapter
C2 - 31584168
AN - SCOPUS:85072939064
SN - 978-1-4939-9876-0
T3 - Methods in Molecular Biology
SP - 253
EP - 264
BT - Horizontal Gene Transfer
A2 - Cruz, Fernando de la
PB - Humana Press
ER -
ID: 234151105