The ancient CYP716 family is a major contributor to the diversification of eudicot triterpenoid biosynthesis
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The ancient CYP716 family is a major contributor to the diversification of eudicot triterpenoid biosynthesis. / Miettinen, Karel; Pollier, Jacob; Buyst, Dieter; Arendt, Philipp; Csuk, René; Sommerwerk, Sven; Moses, Tessa; Mertens, Jan; Sonawane, Prashant D.; Pauwels, Laurens; Aharoni, Asaph; Martins, José; Nelson, David R.; Goossens, Alain.
I: Nature Communications, Bind 8, 14153, 2017.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - The ancient CYP716 family is a major contributor to the diversification of eudicot triterpenoid biosynthesis
AU - Miettinen, Karel
AU - Pollier, Jacob
AU - Buyst, Dieter
AU - Arendt, Philipp
AU - Csuk, René
AU - Sommerwerk, Sven
AU - Moses, Tessa
AU - Mertens, Jan
AU - Sonawane, Prashant D.
AU - Pauwels, Laurens
AU - Aharoni, Asaph
AU - Martins, José
AU - Nelson, David R.
AU - Goossens, Alain
N1 - Publisher Copyright: © 2017 The Author(s).
PY - 2017
Y1 - 2017
N2 - Triterpenoids are widespread bioactive plant defence compounds with potential use as pharmaceuticals, pesticides and other high-value products. Enzymes belonging to the cytochrome P450 family have an essential role in creating the immense structural diversity of triterpenoids across the plant kingdom. However, for many triterpenoid oxidation reactions, the corresponding enzyme remains unknown. Here we characterize CYP716 enzymes from different medicinal plant species by heterologous expression in engineered yeasts and report ten hitherto unreported triterpenoid oxidation activities, including a cyclization reaction, leading to a triterpenoid lactone. Kingdom-wide phylogenetic analysis of over 400 CYP716s from over 200 plant species reveals details of their evolution and suggests that in eudicots the CYP716s evolved specifically towards triterpenoid biosynthesis. Our findings underscore the great potential of CYP716s as a source for generating triterpenoid structural diversity and expand the toolbox available for synthetic biology programmes for sustainable production of bioactive plant triterpenoids.
AB - Triterpenoids are widespread bioactive plant defence compounds with potential use as pharmaceuticals, pesticides and other high-value products. Enzymes belonging to the cytochrome P450 family have an essential role in creating the immense structural diversity of triterpenoids across the plant kingdom. However, for many triterpenoid oxidation reactions, the corresponding enzyme remains unknown. Here we characterize CYP716 enzymes from different medicinal plant species by heterologous expression in engineered yeasts and report ten hitherto unreported triterpenoid oxidation activities, including a cyclization reaction, leading to a triterpenoid lactone. Kingdom-wide phylogenetic analysis of over 400 CYP716s from over 200 plant species reveals details of their evolution and suggests that in eudicots the CYP716s evolved specifically towards triterpenoid biosynthesis. Our findings underscore the great potential of CYP716s as a source for generating triterpenoid structural diversity and expand the toolbox available for synthetic biology programmes for sustainable production of bioactive plant triterpenoids.
U2 - 10.1038/ncomms14153
DO - 10.1038/ncomms14153
M3 - Journal article
C2 - 28165039
AN - SCOPUS:85011693539
VL - 8
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 14153
ER -
ID: 280017095