Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase
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Dokumenter
- Leggio_2015_Structure_and_boosting
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Lytic polysaccharide monooxygenases (LPMOs) are recently discovered enzymes that oxidatively deconstruct polysaccharides. LPMOs are fundamental in the effective utilization of these substrates by bacteria and fungi; moreover, the enzymes have significant industrial importance. We report here the activity, spectroscopy and three-dimensional structure of a starch-active LPMO, a representative of the new CAZy AA13 family. We demonstrate that these enzymes generate aldonic acid-terminated malto-oligosaccharides from retrograded starch and boost significantly the conversion of this recalcitrant substrate to maltose by β-amylase. The detailed structure of the enzyme's active site yields insights into the mechanism of action of this important class of enzymes.
Originalsprog | Engelsk |
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Artikelnummer | 5961 |
Tidsskrift | Nature Communications |
Vol/bind | 6 |
Antal sider | 9 |
ISSN | 2041-1723 |
DOI | |
Status | Udgivet - 2015 |
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