Protoplast Isolation and Transfection in Wheat
Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
Wheat is one of the major staple crops around the world. A transient expression system is crucial for gene functional studies in wheat as stable transfection is still difficult in most cultivars. Protoplasts could serve as a versatile transient expression tool in wheat research. Here, we describe protocols for wheat protoplast isolation and transfection that are enabled by cellulase R-10 and macerozyme R-10 containing enzymatic solution and polyethylene glycol-mediated method, respectively. In addition, we show an example of efficiency evaluation of the emerging base editors in wheat protoplasts. These protocols are of wide use in both conventional gene functional analysis and reagent functionality evaluation of genome editing in wheat.
Originalsprog | Engelsk |
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Titel | Protoplast Technology : Methods and Protocols |
Redaktører | Kan Wang, Feng Zhang |
Antal sider | 11 |
Forlag | Springer |
Publikationsdato | 2022 |
Sider | 131-141 |
DOI | |
Status | Udgivet - 2022 |
Navn | Methods in Molecular Biology |
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Vol/bind | 2464 |
ISSN | 1064-3745 |
Bibliografisk note
Funding Information:
This work was supported by grants from the National Natural Science Foundation of China (31788103) and Novo Nordisk Foundation (NovoCrops; project #2019OC53580).
Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
ID: 337988857