Pathways to defense metabolites and evading fruit bitterness in genus Solanum evolved through 2-oxoglutarate-dependent dioxygenases

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Pathways to defense metabolites and evading fruit bitterness in genus Solanum evolved through 2-oxoglutarate-dependent dioxygenases. / Cárdenas, Pablo D.; Sonawane, Prashant D.; Heinig, Uwe; Jozwiak, Adam; Panda, Sayantan; Abebie, Bekele; Kazachkova, Yana; Pliner, Margarita; Unger, Tamar; Wolf, Dalia; Ofner, Itai; Vilaprinyo, Ester; Meir, Sagit; Davydov, Olga; Gal-on, Amit; Burdman, Saul; Giri, Ashok; Zamir, Dani; Scherf, Tali; Szymanski, Jedrzej; Rogachev, Ilana; Aharoni, Asaph.

I: Nature Communications, Bind 10, Nr. 1, 5169, 2019.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Cárdenas, PD, Sonawane, PD, Heinig, U, Jozwiak, A, Panda, S, Abebie, B, Kazachkova, Y, Pliner, M, Unger, T, Wolf, D, Ofner, I, Vilaprinyo, E, Meir, S, Davydov, O, Gal-on, A, Burdman, S, Giri, A, Zamir, D, Scherf, T, Szymanski, J, Rogachev, I & Aharoni, A 2019, 'Pathways to defense metabolites and evading fruit bitterness in genus Solanum evolved through 2-oxoglutarate-dependent dioxygenases', Nature Communications, bind 10, nr. 1, 5169. https://doi.org/10.1038/s41467-019-13211-4

APA

Cárdenas, P. D., Sonawane, P. D., Heinig, U., Jozwiak, A., Panda, S., Abebie, B., Kazachkova, Y., Pliner, M., Unger, T., Wolf, D., Ofner, I., Vilaprinyo, E., Meir, S., Davydov, O., Gal-on, A., Burdman, S., Giri, A., Zamir, D., Scherf, T., ... Aharoni, A. (2019). Pathways to defense metabolites and evading fruit bitterness in genus Solanum evolved through 2-oxoglutarate-dependent dioxygenases. Nature Communications, 10(1), [5169]. https://doi.org/10.1038/s41467-019-13211-4

Vancouver

Cárdenas PD, Sonawane PD, Heinig U, Jozwiak A, Panda S, Abebie B o.a. Pathways to defense metabolites and evading fruit bitterness in genus Solanum evolved through 2-oxoglutarate-dependent dioxygenases. Nature Communications. 2019;10(1). 5169. https://doi.org/10.1038/s41467-019-13211-4

Author

Cárdenas, Pablo D. ; Sonawane, Prashant D. ; Heinig, Uwe ; Jozwiak, Adam ; Panda, Sayantan ; Abebie, Bekele ; Kazachkova, Yana ; Pliner, Margarita ; Unger, Tamar ; Wolf, Dalia ; Ofner, Itai ; Vilaprinyo, Ester ; Meir, Sagit ; Davydov, Olga ; Gal-on, Amit ; Burdman, Saul ; Giri, Ashok ; Zamir, Dani ; Scherf, Tali ; Szymanski, Jedrzej ; Rogachev, Ilana ; Aharoni, Asaph. / Pathways to defense metabolites and evading fruit bitterness in genus Solanum evolved through 2-oxoglutarate-dependent dioxygenases. I: Nature Communications. 2019 ; Bind 10, Nr. 1.

Bibtex

@article{498b7a6fa5ae4a839bca039151d7ec4c,
title = "Pathways to defense metabolites and evading fruit bitterness in genus Solanum evolved through 2-oxoglutarate-dependent dioxygenases",
abstract = "The genus Solanum comprises three food crops (potato, tomato, and eggplant), which are consumed on daily basis worldwide and also producers of notorious anti-nutritional steroidal glycoalkaloids (SGAs). Hydroxylated SGAs (i.e. leptinines) serve as precursors for leptines that act as defenses against Colorado Potato Beetle (Leptinotarsa decemlineata Say), an important pest of potato worldwide. However, SGA hydroxylating enzymes remain unknown. Here, we discover that 2-OXOGLUTARATE-DEPENDENT-DIOXYGENASE (2-ODD) enzymes catalyze SGA-hydroxylation across various Solanum species. In contrast to cultivated potato, Solanum chacoense, a widespread wild potato species, has evolved a 2-ODD enzyme leading to the formation of leptinines. Furthermore, we find a related 2-ODD in tomato that catalyzes the hydroxylation of the bitter α-tomatine to hydroxytomatine, the first committed step in the chemical shift towards downstream ripening-associated non-bitter SGAs (e.g. esculeoside A). This 2-ODD enzyme prevents bitterness in ripe tomato fruit consumed today which otherwise would remain unpleasant in taste and more toxic.",
author = "C{\'a}rdenas, {Pablo D.} and Sonawane, {Prashant D.} and Uwe Heinig and Adam Jozwiak and Sayantan Panda and Bekele Abebie and Yana Kazachkova and Margarita Pliner and Tamar Unger and Dalia Wolf and Itai Ofner and Ester Vilaprinyo and Sagit Meir and Olga Davydov and Amit Gal-on and Saul Burdman and Ashok Giri and Dani Zamir and Tali Scherf and Jedrzej Szymanski and Ilana Rogachev and Asaph Aharoni",
year = "2019",
doi = "10.1038/s41467-019-13211-4",
language = "English",
volume = "10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Pathways to defense metabolites and evading fruit bitterness in genus Solanum evolved through 2-oxoglutarate-dependent dioxygenases

AU - Cárdenas, Pablo D.

AU - Sonawane, Prashant D.

AU - Heinig, Uwe

AU - Jozwiak, Adam

AU - Panda, Sayantan

AU - Abebie, Bekele

AU - Kazachkova, Yana

AU - Pliner, Margarita

AU - Unger, Tamar

AU - Wolf, Dalia

AU - Ofner, Itai

AU - Vilaprinyo, Ester

AU - Meir, Sagit

AU - Davydov, Olga

AU - Gal-on, Amit

AU - Burdman, Saul

AU - Giri, Ashok

AU - Zamir, Dani

AU - Scherf, Tali

AU - Szymanski, Jedrzej

AU - Rogachev, Ilana

AU - Aharoni, Asaph

PY - 2019

Y1 - 2019

N2 - The genus Solanum comprises three food crops (potato, tomato, and eggplant), which are consumed on daily basis worldwide and also producers of notorious anti-nutritional steroidal glycoalkaloids (SGAs). Hydroxylated SGAs (i.e. leptinines) serve as precursors for leptines that act as defenses against Colorado Potato Beetle (Leptinotarsa decemlineata Say), an important pest of potato worldwide. However, SGA hydroxylating enzymes remain unknown. Here, we discover that 2-OXOGLUTARATE-DEPENDENT-DIOXYGENASE (2-ODD) enzymes catalyze SGA-hydroxylation across various Solanum species. In contrast to cultivated potato, Solanum chacoense, a widespread wild potato species, has evolved a 2-ODD enzyme leading to the formation of leptinines. Furthermore, we find a related 2-ODD in tomato that catalyzes the hydroxylation of the bitter α-tomatine to hydroxytomatine, the first committed step in the chemical shift towards downstream ripening-associated non-bitter SGAs (e.g. esculeoside A). This 2-ODD enzyme prevents bitterness in ripe tomato fruit consumed today which otherwise would remain unpleasant in taste and more toxic.

AB - The genus Solanum comprises three food crops (potato, tomato, and eggplant), which are consumed on daily basis worldwide and also producers of notorious anti-nutritional steroidal glycoalkaloids (SGAs). Hydroxylated SGAs (i.e. leptinines) serve as precursors for leptines that act as defenses against Colorado Potato Beetle (Leptinotarsa decemlineata Say), an important pest of potato worldwide. However, SGA hydroxylating enzymes remain unknown. Here, we discover that 2-OXOGLUTARATE-DEPENDENT-DIOXYGENASE (2-ODD) enzymes catalyze SGA-hydroxylation across various Solanum species. In contrast to cultivated potato, Solanum chacoense, a widespread wild potato species, has evolved a 2-ODD enzyme leading to the formation of leptinines. Furthermore, we find a related 2-ODD in tomato that catalyzes the hydroxylation of the bitter α-tomatine to hydroxytomatine, the first committed step in the chemical shift towards downstream ripening-associated non-bitter SGAs (e.g. esculeoside A). This 2-ODD enzyme prevents bitterness in ripe tomato fruit consumed today which otherwise would remain unpleasant in taste and more toxic.

U2 - 10.1038/s41467-019-13211-4

DO - 10.1038/s41467-019-13211-4

M3 - Journal article

C2 - 31727889

AN - SCOPUS:85075034686

VL - 10

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 5169

ER -

ID: 234142548