TY - JOUR

T1 - Partial characterization of Maize rayado fino virus isolates from Ecuador

T2 - phylogenetic analysis supports a Central American origin of the virus

AU - Chicas, Mauricio

AU - Caviedes, Mario

AU - Hammond, Rosemarie

AU - Madriz Ordenana, Kenneth

AU - Albertazzi, Federico

AU - Villalobos, Heydi

AU - Ramírez, Pilar

PY - 2007

Y1 - 2007

N2 - Maize rayado fino virus (MRFV) infects maize and appears to be restricted to, yet widespread in, the Americas. MRFV was previously unreported from Ecuador. Maize plants exhibiting symptoms of MRFV infection were collected at the Santa Catalina experiment station in Quito, Ecuador. RT-PCR reactions were performed on total RNA extracted from the symptomatic leaves using primers specific for the capsid protein (CP) gene and 3′ non-translated region of MRFV and first strand cDNA as a template. Nucleotide sequence comparisons to previously sequenced MRFV isolates from other geographic regions revealed 88-91% sequence identity. Phylogenetic trees constructed using Maximum Likelihood, UPGMA, Minimal Evolution, Neighbor Joining, and Maximum Parsimony methods separated the MRFV isolates into four groups. These groups may represent geographic isolation generated by the mountainous chains of the American continent. Analysis of the sequences and the genetic distances among the different isolates suggests that MRFV may have originated in Mexico and/or Guatemala and from there it dispersed to the rest of the Americas.

AB - Maize rayado fino virus (MRFV) infects maize and appears to be restricted to, yet widespread in, the Americas. MRFV was previously unreported from Ecuador. Maize plants exhibiting symptoms of MRFV infection were collected at the Santa Catalina experiment station in Quito, Ecuador. RT-PCR reactions were performed on total RNA extracted from the symptomatic leaves using primers specific for the capsid protein (CP) gene and 3′ non-translated region of MRFV and first strand cDNA as a template. Nucleotide sequence comparisons to previously sequenced MRFV isolates from other geographic regions revealed 88-91% sequence identity. Phylogenetic trees constructed using Maximum Likelihood, UPGMA, Minimal Evolution, Neighbor Joining, and Maximum Parsimony methods separated the MRFV isolates into four groups. These groups may represent geographic isolation generated by the mountainous chains of the American continent. Analysis of the sequences and the genetic distances among the different isolates suggests that MRFV may have originated in Mexico and/or Guatemala and from there it dispersed to the rest of the Americas.

KW - Central America

KW - MRFV

KW - Maize rayado fino virus

KW - Marafivirus

U2 - 10.1016/j.virusres.2007.02.011

DO - 10.1016/j.virusres.2007.02.011

M3 - Journal article

C2 - 17386956

VL - 126

SP - 268

EP - 276

JO - Publishers Weekly

JF - Publishers Weekly

SN - 0000-0019

IS - 1-2

ER -