TY - JOUR

T1 - Osteoblastic response to pectin nanocoating on titanium surfaces

AU - Gurzawska, Katarzyna Aleksandra

AU - Svava, Rikke

AU - Yihua, Yu

AU - Haugshøj, Kenneth Brian

AU - Dirscherl, Kai

AU - Levery, Steven Bruce

AU - Chrestensen, Inge Byg

AU - Damager, Iben

AU - Weiss Nielsen, Martin

AU - Jørgensen, Bodil

AU - Jørgensen, Niklas Rye

AU - Gotfredsen, Klaus

PY - 2014

Y1 - 2014

N2 - Osseointegration of titanium implants can be improved by organic and inorganic nanocoating of the surface. The aim of our study was to evaluate the effect of organic nanocoating of titanium surface with unmodified and modified pectin Rhamnogalacturonan-Is (RG-Is) isolated from potato and apple with respect to surface properties and osteogenic response in osteoblastic cells. Nanocoatings on titanium surfaces were evaluated by scanning electron microscopy, contact angle measurements, atomic force microscopy, and X-ray photoelectron spectroscopy. The effect of coated RG-Is on cell adhesion, cell viability, bone matrix formation and mineralization was tested using SaOS-2 cells. Nanocoating with pectin RG-Is affected surface properties and in consequence changed the environment for cellular response. The cells cultured on surfaces coated with RG-Is from potato with high content of linear 1.4-linked galactose produced higher level of mineralized matrix compared with control surfaces and surfaces coated with RG-I with low content of linear 1.4-linked galactose. The study showed that the pectin RG-Is nanocoating not only changed chemical and physical titanium surface properties, but also specific coating with RG-Is containing high amount of galactan increased mineralized matrix formation of osteoblastic cells in vitro.

AB - Osseointegration of titanium implants can be improved by organic and inorganic nanocoating of the surface. The aim of our study was to evaluate the effect of organic nanocoating of titanium surface with unmodified and modified pectin Rhamnogalacturonan-Is (RG-Is) isolated from potato and apple with respect to surface properties and osteogenic response in osteoblastic cells. Nanocoatings on titanium surfaces were evaluated by scanning electron microscopy, contact angle measurements, atomic force microscopy, and X-ray photoelectron spectroscopy. The effect of coated RG-Is on cell adhesion, cell viability, bone matrix formation and mineralization was tested using SaOS-2 cells. Nanocoating with pectin RG-Is affected surface properties and in consequence changed the environment for cellular response. The cells cultured on surfaces coated with RG-Is from potato with high content of linear 1.4-linked galactose produced higher level of mineralized matrix compared with control surfaces and surfaces coated with RG-I with low content of linear 1.4-linked galactose. The study showed that the pectin RG-Is nanocoating not only changed chemical and physical titanium surface properties, but also specific coating with RG-Is containing high amount of galactan increased mineralized matrix formation of osteoblastic cells in vitro.

KW - Nanocoatings

KW - Osseointegration

KW - Osteoblasts

KW - Rhamnogalacturonan-I

KW - Surface properties

KW - Titanium

U2 - 10.1016/j.msec.2014.06.028

DO - 10.1016/j.msec.2014.06.028

M3 - Journal article

C2 - 25175196

AN - SCOPUS:84904720627

VL - 43

SP - 117

EP - 125

JO - Materials Science and Engineering C: Materials for Biological Applications

JF - Materials Science and Engineering C: Materials for Biological Applications

SN - 1873-0191

ER -