Microarray glycan profiling reveals algal fucoidan epitopes in diverse marine metazoans
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Microarray glycan profiling reveals algal fucoidan epitopes in diverse marine metazoans. / Asunción Salmeán, Armando; Hervé, Cécile; Jørgensen, Bodil; Willats, William George Tycho; Mravec, Jozef.
I: Frontiers in Marine Science, Bind 4, 293, 2017.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Microarray glycan profiling reveals algal fucoidan epitopes in diverse marine metazoans
AU - Asunción Salmeán, Armando
AU - Hervé, Cécile
AU - Jørgensen, Bodil
AU - Willats, William George Tycho
AU - Mravec, Jozef
PY - 2017
Y1 - 2017
N2 - Despite the biological importance and pharmacological potential of glycans from marine organisms, there are many unanswered questions regarding their distribution, function, and evolution. Here we describe microarray-based glycan profiling of a diverse selection of marine animals using antibodies raised against fucoidan isolated from a brown alga. We demonstrate the presence of two fucoidan epitopes in six animals belonging to three phyla including Porifera, Molusca, and Chordata. We studied the spatial distribution of these epitopes in Cliona celata ("boring sponge") and identified their restricted localization on the surface of internal chambers. Our results show the potential of high-throughput screening and probes commonly used in plant and algal cell wall biology to study the diversity and distribution of glycan structures in metazoans.
AB - Despite the biological importance and pharmacological potential of glycans from marine organisms, there are many unanswered questions regarding their distribution, function, and evolution. Here we describe microarray-based glycan profiling of a diverse selection of marine animals using antibodies raised against fucoidan isolated from a brown alga. We demonstrate the presence of two fucoidan epitopes in six animals belonging to three phyla including Porifera, Molusca, and Chordata. We studied the spatial distribution of these epitopes in Cliona celata ("boring sponge") and identified their restricted localization on the surface of internal chambers. Our results show the potential of high-throughput screening and probes commonly used in plant and algal cell wall biology to study the diversity and distribution of glycan structures in metazoans.
KW - Anti-glycan antibodies
KW - Cliona celata
KW - Fucoidans
KW - Immunolocalisation
KW - Marine fauna
KW - Microarray profiling
KW - Sponges
KW - Structural polysaccharides
U2 - 10.3389/fmars.2017.00293
DO - 10.3389/fmars.2017.00293
M3 - Journal article
AN - SCOPUS:85029209941
VL - 4
JO - Frontiers in Marine Science
JF - Frontiers in Marine Science
SN - 2296-7745
M1 - 293
ER -
ID: 193505048