Liquid scintillation counting can underestimate 14C-activity of 14CO2 trapped in NaOH

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Standard

Liquid scintillation counting can underestimate 14C-activity of 14CO2 trapped in NaOH. / Boos, Elinor F.; Magid, Jakob; Bruun, Sander; Jørgensen, Niels O.G.

I: Soil Biology and Biochemistry, Bind 166, 108576, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Boos, EF, Magid, J, Bruun, S & Jørgensen, NOG 2022, 'Liquid scintillation counting can underestimate 14C-activity of 14CO2 trapped in NaOH', Soil Biology and Biochemistry, bind 166, 108576. https://doi.org/10.1016/j.soilbio.2022.108576

APA

Boos, E. F., Magid, J., Bruun, S., & Jørgensen, N. O. G. (2022). Liquid scintillation counting can underestimate 14C-activity of 14CO2 trapped in NaOH. Soil Biology and Biochemistry, 166, [108576]. https://doi.org/10.1016/j.soilbio.2022.108576

Vancouver

Boos EF, Magid J, Bruun S, Jørgensen NOG. Liquid scintillation counting can underestimate 14C-activity of 14CO2 trapped in NaOH. Soil Biology and Biochemistry. 2022;166. 108576. https://doi.org/10.1016/j.soilbio.2022.108576

Author

Boos, Elinor F. ; Magid, Jakob ; Bruun, Sander ; Jørgensen, Niels O.G. / Liquid scintillation counting can underestimate 14C-activity of 14CO2 trapped in NaOH. I: Soil Biology and Biochemistry. 2022 ; Bind 166.

Bibtex

@article{f30c0b133b1d43a297d1c03bbf4203d6,
title = "Liquid scintillation counting can underestimate 14C-activity of 14CO2 trapped in NaOH",
abstract = "Mineralization of organic matter in environmental samples is frequently quantified by trapping 14CO2 released from the degradation of 14C-labelled compounds. However, when 14CO2 trapped in NaOH is quantified by liquid scintillation counting, 14C-activity can be gradually lost from the scintillation vials. When combining different molarities and volumes of NaOH and five commercial scintillation cocktails, we observed in some mixtures a rapid loss in 14C-activity of up to 10% after 0.5 h and 60% after 96 h, while other mixtures showed no loss of 14C-activity for at least 96 h. The 14C-activity loss depended on molarity, volume, and CO2 saturation of NaOH, as well as the scintillation cocktail used and the mixing ratio of the two components. We show that the decline of 14C-activity may be caused by the loss of 14CO2 from the scintillation vials. The loss of 14C-activity must be prevented and therefore we provide recommendations how to accurately quantify 14C-activity of 14CO2 trapped in NaOH using liquid scintillation counting.",
keywords = "C-activity loss, CO, Base trap, Liquid scintillation counting, Scintillation cocktail",
author = "Boos, {Elinor F.} and Jakob Magid and Sander Bruun and J{\o}rgensen, {Niels O.G.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
doi = "10.1016/j.soilbio.2022.108576",
language = "English",
volume = "166",
journal = "Soil Biology & Biochemistry",
issn = "0038-0717",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Liquid scintillation counting can underestimate 14C-activity of 14CO2 trapped in NaOH

AU - Boos, Elinor F.

AU - Magid, Jakob

AU - Bruun, Sander

AU - Jørgensen, Niels O.G.

N1 - Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - Mineralization of organic matter in environmental samples is frequently quantified by trapping 14CO2 released from the degradation of 14C-labelled compounds. However, when 14CO2 trapped in NaOH is quantified by liquid scintillation counting, 14C-activity can be gradually lost from the scintillation vials. When combining different molarities and volumes of NaOH and five commercial scintillation cocktails, we observed in some mixtures a rapid loss in 14C-activity of up to 10% after 0.5 h and 60% after 96 h, while other mixtures showed no loss of 14C-activity for at least 96 h. The 14C-activity loss depended on molarity, volume, and CO2 saturation of NaOH, as well as the scintillation cocktail used and the mixing ratio of the two components. We show that the decline of 14C-activity may be caused by the loss of 14CO2 from the scintillation vials. The loss of 14C-activity must be prevented and therefore we provide recommendations how to accurately quantify 14C-activity of 14CO2 trapped in NaOH using liquid scintillation counting.

AB - Mineralization of organic matter in environmental samples is frequently quantified by trapping 14CO2 released from the degradation of 14C-labelled compounds. However, when 14CO2 trapped in NaOH is quantified by liquid scintillation counting, 14C-activity can be gradually lost from the scintillation vials. When combining different molarities and volumes of NaOH and five commercial scintillation cocktails, we observed in some mixtures a rapid loss in 14C-activity of up to 10% after 0.5 h and 60% after 96 h, while other mixtures showed no loss of 14C-activity for at least 96 h. The 14C-activity loss depended on molarity, volume, and CO2 saturation of NaOH, as well as the scintillation cocktail used and the mixing ratio of the two components. We show that the decline of 14C-activity may be caused by the loss of 14CO2 from the scintillation vials. The loss of 14C-activity must be prevented and therefore we provide recommendations how to accurately quantify 14C-activity of 14CO2 trapped in NaOH using liquid scintillation counting.

KW - C-activity loss

KW - CO

KW - Base trap

KW - Liquid scintillation counting

KW - Scintillation cocktail

U2 - 10.1016/j.soilbio.2022.108576

DO - 10.1016/j.soilbio.2022.108576

M3 - Journal article

AN - SCOPUS:85123883651

VL - 166

JO - Soil Biology & Biochemistry

JF - Soil Biology & Biochemistry

SN - 0038-0717

M1 - 108576

ER -

ID: 300914940