Lectin Activity in Commonly Consumed Plant-Based Foods: Calling for Method Harmonization and Risk Assessment
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Lectin Activity in Commonly Consumed Plant-Based Foods : Calling for Method Harmonization and Risk Assessment. / Adamcova, Anezka; Laursen, Kristian Holst; Ballin, Nicolai Zederkopff.
I: Foods, Bind 10, Nr. 11, 2796, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Lectin Activity in Commonly Consumed Plant-Based Foods
T2 - Calling for Method Harmonization and Risk Assessment
AU - Adamcova, Anezka
AU - Laursen, Kristian Holst
AU - Ballin, Nicolai Zederkopff
PY - 2021
Y1 - 2021
N2 - Lectins are ubiquitous proteins characterized through their ability to bind different types of carbohydrates. It is well known that active lectins from insufficiently prepared legumes can cause adverse human health effects. The objective of this study was to determine the activity of lectins in samples across plant families representing commercially available edible plants, and the feasibility of inactivating lectins through soaking and boiling. Lectins were extracted from the plant families Adoxaceae, Amaranthaceae, Cannabaceae, Fabaceae, Gramineae, Lamiaceae, Linaceae, Pedaliaceae, and Solanaceae. A hemagglutination assay based on non-treated or trypsin treated rabbit erythrocytes was used to measure the lectin activity. The results showed the highest lectin activity in species from the Fabaceae family and demonstrated that soaking and boiling have an effect on the levels of active lectins. This is the first large study that combines lectin activity obtained from two different assays with raw and processed edible plants. In addition, we examined the current risk assessment, and regulations necessary for an adequate official reporting of results. We encourage the scientific community to further explore this field and agree on harmonized methods for analysis and interpretation, and hope that our methodology can initiate this development.
AB - Lectins are ubiquitous proteins characterized through their ability to bind different types of carbohydrates. It is well known that active lectins from insufficiently prepared legumes can cause adverse human health effects. The objective of this study was to determine the activity of lectins in samples across plant families representing commercially available edible plants, and the feasibility of inactivating lectins through soaking and boiling. Lectins were extracted from the plant families Adoxaceae, Amaranthaceae, Cannabaceae, Fabaceae, Gramineae, Lamiaceae, Linaceae, Pedaliaceae, and Solanaceae. A hemagglutination assay based on non-treated or trypsin treated rabbit erythrocytes was used to measure the lectin activity. The results showed the highest lectin activity in species from the Fabaceae family and demonstrated that soaking and boiling have an effect on the levels of active lectins. This is the first large study that combines lectin activity obtained from two different assays with raw and processed edible plants. In addition, we examined the current risk assessment, and regulations necessary for an adequate official reporting of results. We encourage the scientific community to further explore this field and agree on harmonized methods for analysis and interpretation, and hope that our methodology can initiate this development.
KW - active lectins
KW - disease
KW - hemagglutination
KW - lectins
KW - legumes
KW - plant-based foods
KW - risk assessment
KW - PHASEOLUS-VULGARIS LECTINS
KW - SOYBEAN LECTIN
KW - PROTEINS
KW - LEGUMES
KW - CONSUMPTION
KW - SURVIVAL
KW - GROWTH
KW - FRUIT
U2 - 10.3390/foods10112796
DO - 10.3390/foods10112796
M3 - Journal article
C2 - 34829077
VL - 10
JO - Foods
JF - Foods
SN - 2304-8158
IS - 11
M1 - 2796
ER -
ID: 287118239