In vitro characterization of the Ac locus in white clover (Trifolium repens L.)
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. l-Valine, 2-methylpropanal oxime, and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. l-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least two steps in these conversions are missing in microsomes from plants of genotype ac ac.
Originalsprog | Engelsk |
---|---|
Tidsskrift | Archives of Biochemistry and Biophysics |
Vol/bind | 218 |
Udgave nummer | 1 |
Sider (fra-til) | 38-45 |
Antal sider | 8 |
ISSN | 0003-9861 |
DOI | |
Status | Udgivet - 1 okt. 1982 |
ID: 201511980