Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures
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Bicelles are disk-shaped models of cellular membranes used to study lipid–protein interactions, as well as for structural and functional studies on transmembrane proteins. One challenge for the incorporation of transmembrane proteins in bicelles is the limited range of detergent and lipid combinations available for the successful reconstitution of proteins in model membranes. This is important, as the function and stability of transmembrane proteins are very closely linked to the detergents used for their purification and to the lipids that the proteins are embedded in. Here, we expand the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. We use a combination of dynamic light scattering, small-angle X-ray scattering and cryogenic electron microscopy to perform a systematic sample characterization, thus providing a set of conditions under which bicelles can be successfully formed.
Originalsprog | Engelsk |
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Artikelnummer | 7628 |
Tidsskrift | Molecules |
Vol/bind | 27 |
Udgave nummer | 21 |
Antal sider | 13 |
ISSN | 1420-3049 |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
This research was funded by Swedish Research Council grant numbers 2018-03990 and 2018-04833 as well as the Wenner-Gren foundation grant number SSh2020-0005.
Publisher Copyright:
© 2022 by the authors.
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