Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation

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Standard

Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation. / Libiakova, G.; Jørgensen, B.; Palmgren, G.; Ulvskov, P.; Johansen, E.

I: Plant Cell Reports, Bind 20, Nr. 7, 2001, s. 610-615.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Libiakova, G, Jørgensen, B, Palmgren, G, Ulvskov, P & Johansen, E 2001, 'Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation', Plant Cell Reports, bind 20, nr. 7, s. 610-615. https://doi.org/10.1007/s002990100375

APA

Libiakova, G., Jørgensen, B., Palmgren, G., Ulvskov, P., & Johansen, E. (2001). Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation. Plant Cell Reports, 20(7), 610-615. https://doi.org/10.1007/s002990100375

Vancouver

Libiakova G, Jørgensen B, Palmgren G, Ulvskov P, Johansen E. Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation. Plant Cell Reports. 2001;20(7):610-615. https://doi.org/10.1007/s002990100375

Author

Libiakova, G. ; Jørgensen, B. ; Palmgren, G. ; Ulvskov, P. ; Johansen, E. / Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation. I: Plant Cell Reports. 2001 ; Bind 20, Nr. 7. s. 610-615.

Bibtex

@article{a8fa38117f9544519960b1d49f0da1f4,
title = "Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation",
abstract = "In this project we have analysed the use of an intron-containing neomycin phosphotransferase II - nptII - gene. The advantage of this construct is that only eukaryotic organisms will be able to process this gene. Accordingly, the theoretical risk of horizontal gene flow of antibiotic resistance genes from transgenic plants to enteric bacteria is eliminated. The ST-LS1 intron IV2 from potato was inserted into the coding region of nptII. Transformation of Solanum tuberosum (potato) and Nicotiana tabacum (tobacco) with constructs containing the intron nptII showed similar transformation frequencies to transformation with constructs containing the normal nptII. Analysis of total DNA and RNA confirmed that the intron-containing nptII gene was present in the plants and that the mRNA was processed correctly.",
keywords = "Intron, NPTII, Potato, Selection, Tobacco",
author = "G. Libiakova and B. J{\o}rgensen and G. Palmgren and P. Ulvskov and E. Johansen",
note = "Funding Information: Acknowledgements The work was performed during the stay of Gabriela Libiakova as guest researcher in the Biotechnology Group, DIAS, and was supported by the Danish Academy of Research Danvis project 1997–145–004. The authors would like to thank Ms Winnie Dam for helping with the tissue culture work.",
year = "2001",
doi = "10.1007/s002990100375",
language = "English",
volume = "20",
pages = "610--615",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer",
number = "7",

}

RIS

TY - JOUR

T1 - Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation

AU - Libiakova, G.

AU - Jørgensen, B.

AU - Palmgren, G.

AU - Ulvskov, P.

AU - Johansen, E.

N1 - Funding Information: Acknowledgements The work was performed during the stay of Gabriela Libiakova as guest researcher in the Biotechnology Group, DIAS, and was supported by the Danish Academy of Research Danvis project 1997–145–004. The authors would like to thank Ms Winnie Dam for helping with the tissue culture work.

PY - 2001

Y1 - 2001

N2 - In this project we have analysed the use of an intron-containing neomycin phosphotransferase II - nptII - gene. The advantage of this construct is that only eukaryotic organisms will be able to process this gene. Accordingly, the theoretical risk of horizontal gene flow of antibiotic resistance genes from transgenic plants to enteric bacteria is eliminated. The ST-LS1 intron IV2 from potato was inserted into the coding region of nptII. Transformation of Solanum tuberosum (potato) and Nicotiana tabacum (tobacco) with constructs containing the intron nptII showed similar transformation frequencies to transformation with constructs containing the normal nptII. Analysis of total DNA and RNA confirmed that the intron-containing nptII gene was present in the plants and that the mRNA was processed correctly.

AB - In this project we have analysed the use of an intron-containing neomycin phosphotransferase II - nptII - gene. The advantage of this construct is that only eukaryotic organisms will be able to process this gene. Accordingly, the theoretical risk of horizontal gene flow of antibiotic resistance genes from transgenic plants to enteric bacteria is eliminated. The ST-LS1 intron IV2 from potato was inserted into the coding region of nptII. Transformation of Solanum tuberosum (potato) and Nicotiana tabacum (tobacco) with constructs containing the intron nptII showed similar transformation frequencies to transformation with constructs containing the normal nptII. Analysis of total DNA and RNA confirmed that the intron-containing nptII gene was present in the plants and that the mRNA was processed correctly.

KW - Intron

KW - NPTII

KW - Potato

KW - Selection

KW - Tobacco

UR - http://www.scopus.com/inward/record.url?scp=0034776641&partnerID=8YFLogxK

U2 - 10.1007/s002990100375

DO - 10.1007/s002990100375

M3 - Journal article

AN - SCOPUS:0034776641

VL - 20

SP - 610

EP - 615

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 7

ER -

ID: 308329209