Detection of legumin gene DNA sequences in pea by in situ hybridization

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Standard

Detection of legumin gene DNA sequences in pea by in situ hybridization. / Simpson, Paul R.; Newman, Mari Anne; Davies, D. Roy.

I: Chromosoma, Bind 96, Nr. 6, 07.1988, s. 454-458.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Simpson, PR, Newman, MA & Davies, DR 1988, 'Detection of legumin gene DNA sequences in pea by in situ hybridization', Chromosoma, bind 96, nr. 6, s. 454-458. https://doi.org/10.1007/BF00303040

APA

Simpson, P. R., Newman, M. A., & Davies, D. R. (1988). Detection of legumin gene DNA sequences in pea by in situ hybridization. Chromosoma, 96(6), 454-458. https://doi.org/10.1007/BF00303040

Vancouver

Simpson PR, Newman MA, Davies DR. Detection of legumin gene DNA sequences in pea by in situ hybridization. Chromosoma. 1988 jul.;96(6):454-458. https://doi.org/10.1007/BF00303040

Author

Simpson, Paul R. ; Newman, Mari Anne ; Davies, D. Roy. / Detection of legumin gene DNA sequences in pea by in situ hybridization. I: Chromosoma. 1988 ; Bind 96, Nr. 6. s. 454-458.

Bibtex

@article{45f9db9c5c9845bba940d40028d401ad,
title = "Detection of legumin gene DNA sequences in pea by in situ hybridization",
abstract = "In order to detect low copy number sequences in pea using biotin-labelled probes we optimised some aspects of the in situ hybridization technique. We found protoplast preparations to be superior to standard squashes in terms of their signal: noise ratio. Heat and alkali denaturation of chromosomal DNA were both more effective than acid denaturation. A comparison of antibody-fluorochrome and streptavidin-enzyme conjugates showed the streptavidin-alkaline phosphatase conjugate to be the most sensitive detection system. Using the optimised method, we were able to detect a single site for a 13.5 kb legumin gene clone.",
author = "Simpson, {Paul R.} and Newman, {Mari Anne} and Davies, {D. Roy}",
year = "1988",
month = jul,
doi = "10.1007/BF00303040",
language = "English",
volume = "96",
pages = "454--458",
journal = "Chromosoma",
issn = "0009-5915",
publisher = "Springer",
number = "6",

}

RIS

TY - JOUR

T1 - Detection of legumin gene DNA sequences in pea by in situ hybridization

AU - Simpson, Paul R.

AU - Newman, Mari Anne

AU - Davies, D. Roy

PY - 1988/7

Y1 - 1988/7

N2 - In order to detect low copy number sequences in pea using biotin-labelled probes we optimised some aspects of the in situ hybridization technique. We found protoplast preparations to be superior to standard squashes in terms of their signal: noise ratio. Heat and alkali denaturation of chromosomal DNA were both more effective than acid denaturation. A comparison of antibody-fluorochrome and streptavidin-enzyme conjugates showed the streptavidin-alkaline phosphatase conjugate to be the most sensitive detection system. Using the optimised method, we were able to detect a single site for a 13.5 kb legumin gene clone.

AB - In order to detect low copy number sequences in pea using biotin-labelled probes we optimised some aspects of the in situ hybridization technique. We found protoplast preparations to be superior to standard squashes in terms of their signal: noise ratio. Heat and alkali denaturation of chromosomal DNA were both more effective than acid denaturation. A comparison of antibody-fluorochrome and streptavidin-enzyme conjugates showed the streptavidin-alkaline phosphatase conjugate to be the most sensitive detection system. Using the optimised method, we were able to detect a single site for a 13.5 kb legumin gene clone.

UR - http://www.scopus.com/inward/record.url?scp=0001006430&partnerID=8YFLogxK

U2 - 10.1007/BF00303040

DO - 10.1007/BF00303040

M3 - Journal article

AN - SCOPUS:0001006430

VL - 96

SP - 454

EP - 458

JO - Chromosoma

JF - Chromosoma

SN - 0009-5915

IS - 6

ER -

ID: 380059122