Comparative Metabolomics and Molecular Phylogenetics of Melon (Cucumis melo, Cucurbitaceae) Biodiversity
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
Comparative Metabolomics and Molecular Phylogenetics of Melon (Cucumis melo, Cucurbitaceae) Biodiversity. / Moing, Annick; Allwood, J. William; Aharoni, Asaph; Baker, John; Beale, Michael H.; Ben-Dor, Shifra; Biais, Benoit; Brigante, Federico; Burger, Yosef; Deborde, Catherine; Erban, Alexander; Faigenboim, Adi; Gur, Amit; Goodacre, Royston; Hansen, Thomas H.; Jacob, Daniel; Katzir, Nurit; Kopka, Joachim; Lewinsohn, Efraim; Maucourt, Mickael; Meir, Sagit; Miller, Sonia; Mumm, Roland; Oren, Elad; Paris, Harry S.; Rogachev, Ilana; Rolin, Dominique; Saar, Uzi; Schjoerring, Jan K.; Tadmor, Yaakov; Tzuri, Galil; de Vos, Ric C. H.; Ward, Jane L.; Yeselson, Elena; Hall, Robert D.; Schaffer, Arthur A.
I: Metabolites, Bind 10, Nr. 3, 121, 2020.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Comparative Metabolomics and Molecular Phylogenetics of Melon (Cucumis melo, Cucurbitaceae) Biodiversity
AU - Moing, Annick
AU - Allwood, J. William
AU - Aharoni, Asaph
AU - Baker, John
AU - Beale, Michael H.
AU - Ben-Dor, Shifra
AU - Biais, Benoit
AU - Brigante, Federico
AU - Burger, Yosef
AU - Deborde, Catherine
AU - Erban, Alexander
AU - Faigenboim, Adi
AU - Gur, Amit
AU - Goodacre, Royston
AU - Hansen, Thomas H.
AU - Jacob, Daniel
AU - Katzir, Nurit
AU - Kopka, Joachim
AU - Lewinsohn, Efraim
AU - Maucourt, Mickael
AU - Meir, Sagit
AU - Miller, Sonia
AU - Mumm, Roland
AU - Oren, Elad
AU - Paris, Harry S.
AU - Rogachev, Ilana
AU - Rolin, Dominique
AU - Saar, Uzi
AU - Schjoerring, Jan K.
AU - Tadmor, Yaakov
AU - Tzuri, Galil
AU - de Vos, Ric C. H.
AU - Ward, Jane L.
AU - Yeselson, Elena
AU - Hall, Robert D.
AU - Schaffer, Arthur A.
PY - 2020
Y1 - 2020
N2 - The broad variability of Cucumis melo (melon, Cucurbitaceae) presents a challenge to conventional classification and organization within the species. To shed further light on the infraspecific relationships within C. melo, we compared genotypic and metabolomic similarities among 44 accessions representative of most of the cultivar-groups. Genotyping-by-sequencing (GBS) provided over 20,000 single-nucleotide polymorphisms (SNPs). Metabolomics data of the mature fruit flesh and rind provided over 80,000 metabolomic and elemental features via an orchestra of six complementary metabolomic platforms. These technologies probed polar, semi-polar, and non-polar metabolite fractions as well as a set of mineral elements and included both flavor- and taste-relevant volatile and non-volatile metabolites. Together these results enabled an estimate of "metabolomic/elemental distance" and its correlation with the genetic GBS distance of melon accessions. This study indicates that extensive and non-targeted metabolomics/elemental characterization produced classifications that strongly, but not completely, reflect the current and extensive genetic classification. Certain melon Groups, such as Inodorous, clustered in parallel with the genetic classifications while other genome to metabolome/element associations proved less clear. We suggest that the combined genomic, metabolic, and element data reflect the extensive sexual compatibility among melon accessions and the breeding history that has, for example, targeted metabolic quality traits, such as taste and flavor.
AB - The broad variability of Cucumis melo (melon, Cucurbitaceae) presents a challenge to conventional classification and organization within the species. To shed further light on the infraspecific relationships within C. melo, we compared genotypic and metabolomic similarities among 44 accessions representative of most of the cultivar-groups. Genotyping-by-sequencing (GBS) provided over 20,000 single-nucleotide polymorphisms (SNPs). Metabolomics data of the mature fruit flesh and rind provided over 80,000 metabolomic and elemental features via an orchestra of six complementary metabolomic platforms. These technologies probed polar, semi-polar, and non-polar metabolite fractions as well as a set of mineral elements and included both flavor- and taste-relevant volatile and non-volatile metabolites. Together these results enabled an estimate of "metabolomic/elemental distance" and its correlation with the genetic GBS distance of melon accessions. This study indicates that extensive and non-targeted metabolomics/elemental characterization produced classifications that strongly, but not completely, reflect the current and extensive genetic classification. Certain melon Groups, such as Inodorous, clustered in parallel with the genetic classifications while other genome to metabolome/element associations proved less clear. We suggest that the combined genomic, metabolic, and element data reflect the extensive sexual compatibility among melon accessions and the breeding history that has, for example, targeted metabolic quality traits, such as taste and flavor.
KW - genetic resources
KW - melon
KW - genotype by sequencing
KW - elemental analysis
KW - metabolome
KW - Cucumis melo
KW - MASS-SPECTROMETRY DATA
KW - FRUIT
KW - CHROMATOGRAPHY
KW - TOOL
U2 - 10.3390/metabo10030121
DO - 10.3390/metabo10030121
M3 - Journal article
C2 - 32213984
VL - 10
JO - Metabolites
JF - Metabolites
SN - 2218-1989
IS - 3
M1 - 121
ER -
ID: 249482274