Barley endosomal MONENSIN SENSITIVITY1 is a target of the powdery mildew effector CSEP0162 and plays a role in plant immunity
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Barley endosomal MONENSIN SENSITIVITY1 is a target of the powdery mildew effector CSEP0162 and plays a role in plant immunity. / Liao, Wenlin; Nielsen, Mads E.; Pedersen, Carsten; Xie, Wenjun; Thordal-Christensen, Hans.
I: Journal of Experimental Botany, Bind 74, Nr. 1, 2023, s. 118-129.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Barley endosomal MONENSIN SENSITIVITY1 is a target of the powdery mildew effector CSEP0162 and plays a role in plant immunity
AU - Liao, Wenlin
AU - Nielsen, Mads E.
AU - Pedersen, Carsten
AU - Xie, Wenjun
AU - Thordal-Christensen, Hans
PY - 2023
Y1 - 2023
N2 - Encasements formed around haustoria and biotrophic hyphae as well as hypersensitive reaction (HR) cell death are essential plant immune responses to filamentous pathogens. In this study we examine the components that may contribute to the absence of these responses in susceptible barley attacked by the powdery mildew fungus. We find that the effector CSEP0162 from this pathogen targets plant MONENSIN SENSITIVITY1 (MON1), which is important for the fusion of multivesicular bodies to their target membranes. Overexpression of CSEP0162 and silencing of barley MON1 both inhibit encasement formation. We find that the Arabidopsis ecotype No-0 has resistance to powdery mildew, and that this is partially dependent on MON1. Surprisingly, we find the MON1-dependent resistance in No-0 not only includes an encasement response, but also an effective HR. Similarly, silencing of MON1 in barley also blocks Mla3-mediated HR-based powdery mildew resistance. Our results indicate that MON1 is a vital plant immunity component, and we speculate that the barley powdery mildew fungus introduces the effector CSEP0162 to target MON1 and hence reduce encasement formation and HR.
AB - Encasements formed around haustoria and biotrophic hyphae as well as hypersensitive reaction (HR) cell death are essential plant immune responses to filamentous pathogens. In this study we examine the components that may contribute to the absence of these responses in susceptible barley attacked by the powdery mildew fungus. We find that the effector CSEP0162 from this pathogen targets plant MONENSIN SENSITIVITY1 (MON1), which is important for the fusion of multivesicular bodies to their target membranes. Overexpression of CSEP0162 and silencing of barley MON1 both inhibit encasement formation. We find that the Arabidopsis ecotype No-0 has resistance to powdery mildew, and that this is partially dependent on MON1. Surprisingly, we find the MON1-dependent resistance in No-0 not only includes an encasement response, but also an effective HR. Similarly, silencing of MON1 in barley also blocks Mla3-mediated HR-based powdery mildew resistance. Our results indicate that MON1 is a vital plant immunity component, and we speculate that the barley powdery mildew fungus introduces the effector CSEP0162 to target MON1 and hence reduce encasement formation and HR.
KW - Barley powdery mildew
KW - Blumeria hordei
KW - effector
KW - encasement
KW - Hordeum vulgare
KW - immunity
KW - MON1
KW - multivesicular body
KW - pathogen resistance
KW - CELL-DEATH
KW - DISEASE RESISTANCE
KW - ARABIDOPSIS
KW - GENES
KW - DEFENSE
KW - GTPASE
KW - PAPILLAE
KW - TETRACONAZOLE
KW - TRAFFICKING
KW - ACTIVATION
U2 - 10.1093/jxb/erac403
DO - 10.1093/jxb/erac403
M3 - Journal article
C2 - 36227010
VL - 74
SP - 118
EP - 129
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
SN - 0022-0957
IS - 1
ER -
ID: 329247294