In vitro Assay to Evaluate Cation Transport of Ionophores
Research output: Contribution to journal › Journal article › Research › peer-review
Ion homeostasis is a fundamental regulator of cellular processes and depends upon lipid membranes, which function as ion permeability barriers. Ionophores facilitate ion transport across cell membranes and offer a way to manipulate cellular ion composition. Here, we describe a calcein quenching assay based on large unilamellar vesicles that we used to evaluate divalent cation transport of the ionophore 4-Br-A23187. This assay can be used to study metal transport by ionophores and membrane proteins, under well-defined conditions.
Original language | English |
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Article number | e4552 |
Journal | Bio-protocol |
Volume | 12 |
Issue number | 22 |
Number of pages | 11 |
ISSN | 2331-8325 |
DOIs | |
Publication status | Published - 2022 |
Links
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9724011/?report=printable
Final published version
ID: 337988383