In vitro Assay to Evaluate Cation Transport of Ionophores

Research output: Contribution to journal › Journal article › Research › peer-review

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In vitro Assay to Evaluate Cation Transport of Ionophores. / Uzun, Huriye; Vázquez-Hernández, Melissa; Bandow, Julia; Pomorski, Thomas.

In: Bio-protocol, Vol. 12, No. 22, e4552, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Uzun, H, Vázquez-Hernández, M, Bandow, J & Pomorski, T 2022, 'In vitro Assay to Evaluate Cation Transport of Ionophores', Bio-protocol, vol. 12, no. 22, e4552. https://doi.org/10.21769/BioProtoc.4552

APA

Uzun, H., Vázquez-Hernández, M., Bandow, J., & Pomorski, T. (2022). In vitro Assay to Evaluate Cation Transport of Ionophores. Bio-protocol, 12(22), [e4552]. https://doi.org/10.21769/BioProtoc.4552

Vancouver

Uzun H, Vázquez-Hernández M, Bandow J, Pomorski T. In vitro Assay to Evaluate Cation Transport of Ionophores. Bio-protocol. 2022;12(22). e4552. https://doi.org/10.21769/BioProtoc.4552

Author

Uzun, Huriye ; Vázquez-Hernández, Melissa ; Bandow, Julia ; Pomorski, Thomas. / In vitro Assay to Evaluate Cation Transport of Ionophores. In: Bio-protocol. 2022 ; Vol. 12, No. 22.

Bibtex

@article{fee45dc81fa54260b7239e50b826532d,
title = "In vitro Assay to Evaluate Cation Transport of Ionophores",
abstract = "Ion homeostasis is a fundamental regulator of cellular processes and depends upon lipid membranes, which function as ion permeability barriers. Ionophores facilitate ion transport across cell membranes and offer a way to manipulate cellular ion composition. Here, we describe a calcein quenching assay based on large unilamellar vesicles that we used to evaluate divalent cation transport of the ionophore 4-Br-A23187. This assay can be used to study metal transport by ionophores and membrane proteins, under well-defined conditions.",
author = "Huriye Uzun and Melissa V{\'a}zquez-Hern{\'a}ndez and Julia Bandow and Thomas Pomorski",
year = "2022",
doi = "10.21769/BioProtoc.4552",
language = "English",
volume = "12",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "22",

}

RIS

TY - JOUR

T1 - In vitro Assay to Evaluate Cation Transport of Ionophores

AU - Uzun, Huriye

AU - Vázquez-Hernández, Melissa

AU - Bandow, Julia

AU - Pomorski, Thomas

PY - 2022

Y1 - 2022

N2 - Ion homeostasis is a fundamental regulator of cellular processes and depends upon lipid membranes, which function as ion permeability barriers. Ionophores facilitate ion transport across cell membranes and offer a way to manipulate cellular ion composition. Here, we describe a calcein quenching assay based on large unilamellar vesicles that we used to evaluate divalent cation transport of the ionophore 4-Br-A23187. This assay can be used to study metal transport by ionophores and membrane proteins, under well-defined conditions.

AB - Ion homeostasis is a fundamental regulator of cellular processes and depends upon lipid membranes, which function as ion permeability barriers. Ionophores facilitate ion transport across cell membranes and offer a way to manipulate cellular ion composition. Here, we describe a calcein quenching assay based on large unilamellar vesicles that we used to evaluate divalent cation transport of the ionophore 4-Br-A23187. This assay can be used to study metal transport by ionophores and membrane proteins, under well-defined conditions.

U2 - 10.21769/BioProtoc.4552

DO - 10.21769/BioProtoc.4552

M3 - Journal article

C2 - 36532682

VL - 12

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 22

M1 - e4552

ER -

ID: 337988383