Cell-based lipid flippase assay employing fluorescent lipid derivatives

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Cell-based lipid flippase assay employing fluorescent lipid derivatives. / Jensen, Maria Stumph; Costa, Sara; Günther-Pomorski, Thomas; Lopez Marques, Rosa Laura.

P-Type ATPases: methods and protocols. ed. / Maike Bublitz. Vol. VI Springer, 2016. p. 371-382 (Methods in Molecular Biology, Vol. 1377).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Jensen, MS, Costa, S, Günther-Pomorski, T & Lopez Marques, RL 2016, Cell-based lipid flippase assay employing fluorescent lipid derivatives. in M Bublitz (ed.), P-Type ATPases: methods and protocols. vol. VI, Springer, Methods in Molecular Biology, vol. 1377, pp. 371-382. https://doi.org/10.1007/978-1-4939-3179-8_33

APA

Jensen, M. S., Costa, S., Günther-Pomorski, T., & Lopez Marques, R. L. (2016). Cell-based lipid flippase assay employing fluorescent lipid derivatives. In M. Bublitz (Ed.), P-Type ATPases: methods and protocols (Vol. VI, pp. 371-382). Springer. Methods in Molecular Biology Vol. 1377 https://doi.org/10.1007/978-1-4939-3179-8_33

Vancouver

Jensen MS, Costa S, Günther-Pomorski T, Lopez Marques RL. Cell-based lipid flippase assay employing fluorescent lipid derivatives. In Bublitz M, editor, P-Type ATPases: methods and protocols. Vol. VI. Springer. 2016. p. 371-382. (Methods in Molecular Biology, Vol. 1377). https://doi.org/10.1007/978-1-4939-3179-8_33

Author

Jensen, Maria Stumph ; Costa, Sara ; Günther-Pomorski, Thomas ; Lopez Marques, Rosa Laura. / Cell-based lipid flippase assay employing fluorescent lipid derivatives. P-Type ATPases: methods and protocols. editor / Maike Bublitz. Vol. VI Springer, 2016. pp. 371-382 (Methods in Molecular Biology, Vol. 1377).

Bibtex

@inbook{15ff1a79717d45f0b7b9dae39db27245,
title = "Cell-based lipid flippase assay employing fluorescent lipid derivatives",
abstract = "P-type ATPases in the P4 subfamily (P4-ATPases) are transmembrane proteins unique for eukaryotes that act as lipid flippases, i.e., to translocate phospholipids from the exofacial to the cytofacial monolayer of cellular membranes. While initially characterized as aminophospholipid translocases, studies of individual P4-ATPase family members from fungi, plants, and animals show that P4-ATPases differ in their substrate specificities and mediate transport of a broader range of lipid substrates. Here, we describe an assay based on fluorescent lipid derivatives to monitor and characterize lipid flippase activities in the plasma membrane of cells, using yeast as an example.",
keywords = "Azoles, Enzyme Assays, Flow Cytometry, Fluorescent Dyes, Lipid Metabolism, Lipids, Nitrobenzenes, Phospholipid Transfer Proteins, Saccharomyces cerevisiae, Journal Article",
author = "Jensen, {Maria Stumph} and Sara Costa and Thomas G{\"u}nther-Pomorski and {Lopez Marques}, {Rosa Laura}",
year = "2016",
doi = "10.1007/978-1-4939-3179-8_33",
language = "English",
isbn = "978-1-4939-3178-1",
volume = "VI",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "371--382",
editor = "Maike Bublitz",
booktitle = "P-Type ATPases",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Cell-based lipid flippase assay employing fluorescent lipid derivatives

AU - Jensen, Maria Stumph

AU - Costa, Sara

AU - Günther-Pomorski, Thomas

AU - Lopez Marques, Rosa Laura

PY - 2016

Y1 - 2016

N2 - P-type ATPases in the P4 subfamily (P4-ATPases) are transmembrane proteins unique for eukaryotes that act as lipid flippases, i.e., to translocate phospholipids from the exofacial to the cytofacial monolayer of cellular membranes. While initially characterized as aminophospholipid translocases, studies of individual P4-ATPase family members from fungi, plants, and animals show that P4-ATPases differ in their substrate specificities and mediate transport of a broader range of lipid substrates. Here, we describe an assay based on fluorescent lipid derivatives to monitor and characterize lipid flippase activities in the plasma membrane of cells, using yeast as an example.

AB - P-type ATPases in the P4 subfamily (P4-ATPases) are transmembrane proteins unique for eukaryotes that act as lipid flippases, i.e., to translocate phospholipids from the exofacial to the cytofacial monolayer of cellular membranes. While initially characterized as aminophospholipid translocases, studies of individual P4-ATPase family members from fungi, plants, and animals show that P4-ATPases differ in their substrate specificities and mediate transport of a broader range of lipid substrates. Here, we describe an assay based on fluorescent lipid derivatives to monitor and characterize lipid flippase activities in the plasma membrane of cells, using yeast as an example.

KW - Azoles

KW - Enzyme Assays

KW - Flow Cytometry

KW - Fluorescent Dyes

KW - Lipid Metabolism

KW - Lipids

KW - Nitrobenzenes

KW - Phospholipid Transfer Proteins

KW - Saccharomyces cerevisiae

KW - Journal Article

U2 - 10.1007/978-1-4939-3179-8_33

DO - 10.1007/978-1-4939-3179-8_33

M3 - Book chapter

C2 - 26695048

SN - 978-1-4939-3178-1

VL - VI

T3 - Methods in Molecular Biology

SP - 371

EP - 382

BT - P-Type ATPases

A2 - Bublitz, Maike

PB - Springer

ER -

ID: 169355616