Cell-based lipid flippase assay employing fluorescent lipid derivatives
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
P-type ATPases in the P4 subfamily (P4-ATPases) are transmembrane proteins unique for eukaryotes that act as lipid flippases, i.e., to translocate phospholipids from the exofacial to the cytofacial monolayer of cellular membranes. While initially characterized as aminophospholipid translocases, studies of individual P4-ATPase family members from fungi, plants, and animals show that P4-ATPases differ in their substrate specificities and mediate transport of a broader range of lipid substrates. Here, we describe an assay based on fluorescent lipid derivatives to monitor and characterize lipid flippase activities in the plasma membrane of cells, using yeast as an example.
Original language | English |
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Title of host publication | P-Type ATPases : methods and protocols |
Editors | Maike Bublitz |
Number of pages | 12 |
Volume | VI |
Publisher | Springer |
Publication date | 2016 |
Pages | 371-382 |
Chapter | 33 |
ISBN (Print) | 978-1-4939-3178-1 |
ISBN (Electronic) | 978-1-4939-3179-8 |
DOIs | |
Publication status | Published - 2016 |
Series | Methods in Molecular Biology |
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Volume | 1377 |
ISSN | 1064-3745 |
- Azoles, Enzyme Assays, Flow Cytometry, Fluorescent Dyes, Lipid Metabolism, Lipids, Nitrobenzenes, Phospholipid Transfer Proteins, Saccharomyces cerevisiae, Journal Article
Research areas
ID: 169355616