Ansatte på Institut for Plante- og Miljøvidenskab – Københavns Universitet

Cecilie Ida Cetti Hansen

Cecilie Ida Cetti Hansen


I study plant specialized metabolism with special focus on the superfamily of cytochromes P450 (P450s) and their redox partner, the NADPH-dependent cytochrome P450 oxidoreductase (POR). Each higher plant species possesses more than hundred different P450s, which carry out fundamental roles in general and specialized metabolism such as the synthesis of essential hormones and bio-active natural products. In contrast, each separate plant species usually possesses 1 to 3 different PORs present in sub-stoichiometric amounts compared to the total level of P450s present. Specific orchestration of the electron transfer processes from a POR to a specific P450 is thus required in the course of plant growth and development and to enable the plant to respond to biotic and abiotic stresses encountered.

Cyanogenic glucosides, a class of amino acid-derived defence compounds, are biosynthesized by the combined action of P450s, PORs and glycosyltransferases. The well-characterized biosynthetic pathway of the cyanogenic glucoside dhurrin from Sorghum bicolor and the newly identified biosynthetic pathway of prunasin from Eucalyptus cladocalyx are used as model systems to study interactions between PORs and P450s.  Special focus is on the assembly and structural organization of the transient enzyme complexes (metabolons) involved. 

ID: 165030639