Isolation and characterisation of a pod dehiscence zone-specific polygalacturonase from Brassica napus
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Isolation and characterisation of a pod dehiscence zone-specific polygalacturonase from Brassica napus. / Petersen, Morten; Sander, Lilli; Child, Robin; Van Onckelen, Harry; Ulvskov, Peter; Borkhardt, Bernhard.
I: Plant Molecular Biology, Bind 31, Nr. 3, 1996, s. 517-527.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Isolation and characterisation of a pod dehiscence zone-specific polygalacturonase from Brassica napus
AU - Petersen, Morten
AU - Sander, Lilli
AU - Child, Robin
AU - Van Onckelen, Harry
AU - Ulvskov, Peter
AU - Borkhardt, Bernhard
PY - 1996
Y1 - 1996
N2 - Seven distinct partial cDNAs, similar in sequence to previously described polygalacturonases (PGs), were amplified from cDNA derived from rape pod wall, dehiscence zone anti leaves by the polymerase chain reaction. Northern analysis showed that one clone, PG35-8, was expressed at low levels in the dehiscence zone during the first five weeks after anthesis but was very abundantly expressed at week 6. In contrast, no PG35-8-related RNA was detected in the pod wall. Our data suggest that there are temporal and spatial correlations between the breakdown of the middle lamella, of the dehiscence zone cells and the pattern of synthesis of PG35-8 transcripts which may indicate a role for this particular PG in rape pod dehiscence. PG35-8 was used to isolate five cDNA clones from a rape dehiscence zone cDNA library. Restriction enzyme analysis and partial sequencing revealed that they were derived from four highly homologous transcripts which are probably allelic forms of a single gene. One full-length clone, RDPG1, was completely sequenced. The predicted protein of RDPG1 showed its highest identity with PG from apple fruit with an identity of 52%.
AB - Seven distinct partial cDNAs, similar in sequence to previously described polygalacturonases (PGs), were amplified from cDNA derived from rape pod wall, dehiscence zone anti leaves by the polymerase chain reaction. Northern analysis showed that one clone, PG35-8, was expressed at low levels in the dehiscence zone during the first five weeks after anthesis but was very abundantly expressed at week 6. In contrast, no PG35-8-related RNA was detected in the pod wall. Our data suggest that there are temporal and spatial correlations between the breakdown of the middle lamella, of the dehiscence zone cells and the pattern of synthesis of PG35-8 transcripts which may indicate a role for this particular PG in rape pod dehiscence. PG35-8 was used to isolate five cDNA clones from a rape dehiscence zone cDNA library. Restriction enzyme analysis and partial sequencing revealed that they were derived from four highly homologous transcripts which are probably allelic forms of a single gene. One full-length clone, RDPG1, was completely sequenced. The predicted protein of RDPG1 showed its highest identity with PG from apple fruit with an identity of 52%.
KW - Brassica napus
KW - pod dehiscence
KW - pod shatter
KW - polygalacturonase isoforms
U2 - 10.1007/BF00042225
DO - 10.1007/BF00042225
M3 - Journal article
C2 - 8790285
AN - SCOPUS:0030175266
VL - 31
SP - 517
EP - 527
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 3
ER -
ID: 308329583