Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici

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Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici. / Schuster, M; Kilaru, S; Latz, Meike Anna Christine; Steinberg, G.

I: Fungal Genetics and Biology, Bind 79, 2015, s. 141-149.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Schuster, M, Kilaru, S, Latz, MAC & Steinberg, G 2015, 'Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici', Fungal Genetics and Biology, bind 79, s. 141-149. https://doi.org/10.1016/j.fgb.2015.03.005

APA

Schuster, M., Kilaru, S., Latz, M. A. C., & Steinberg, G. (2015). Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici. Fungal Genetics and Biology, 79, 141-149. https://doi.org/10.1016/j.fgb.2015.03.005

Vancouver

Schuster M, Kilaru S, Latz MAC, Steinberg G. Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici. Fungal Genetics and Biology. 2015;79:141-149. https://doi.org/10.1016/j.fgb.2015.03.005

Author

Schuster, M ; Kilaru, S ; Latz, Meike Anna Christine ; Steinberg, G. / Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici. I: Fungal Genetics and Biology. 2015 ; Bind 79. s. 141-149.

Bibtex

@article{7206a0b27ee54780ab821c96e13c3911,

title = "Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici",

abstract = "The microtubule cytoskeleton supports vital processes in fungal cells, including hyphal growth and mitosis. Consequently, it is a target for fungicides, such as benomyl. The use of fluorescent fusion proteins to illuminate microtubules and microtubule-associated proteins has led to a break-through in our understanding of their dynamics and function in fungal cells. Here, we introduce fluorescent markers to visualize microtubules and accessory proteins in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein to α-tubulin (ZtTub2), to ZtPeb1, a homologue of the mammalian plus-end binding protein EB1, and to ZtGrc1, a component of the minus-end located γ-tubulin ring complex, involved in the nucleation of microtubules. In vivo observation confirms the localization and dynamic behaviour of all three markers. These marker proteins are useful tools for understanding the organization and importance of the microtubule cytoskeleton in Z. tritici.",

keywords = "Ascomycota, Cytoskeletal Proteins, Genes, Reporter, Luminescent Proteins, Microtubules, Optical Imaging, Recombinant Fusion Proteins, Staining and Labeling",

author = "M Schuster and S Kilaru and Latz, {Meike Anna Christine} and G Steinberg",

note = "Copyright {\textcopyright} 2015. Published by Elsevier Inc.",

year = "2015",

doi = "10.1016/j.fgb.2015.03.005",

language = "English",

volume = "79",

pages = "141--149",

journal = "Fungal Genetics and Biology",

issn = "1087-1845",

publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Fluorescent markers of the microtubule cytoskeleton in Zymoseptoria tritici

AU - Schuster, M

AU - Kilaru, S

AU - Latz, Meike Anna Christine

AU - Steinberg, G

PY - 2015

Y1 - 2015

N2 - The microtubule cytoskeleton supports vital processes in fungal cells, including hyphal growth and mitosis. Consequently, it is a target for fungicides, such as benomyl. The use of fluorescent fusion proteins to illuminate microtubules and microtubule-associated proteins has led to a break-through in our understanding of their dynamics and function in fungal cells. Here, we introduce fluorescent markers to visualize microtubules and accessory proteins in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein to α-tubulin (ZtTub2), to ZtPeb1, a homologue of the mammalian plus-end binding protein EB1, and to ZtGrc1, a component of the minus-end located γ-tubulin ring complex, involved in the nucleation of microtubules. In vivo observation confirms the localization and dynamic behaviour of all three markers. These marker proteins are useful tools for understanding the organization and importance of the microtubule cytoskeleton in Z. tritici.

AB - The microtubule cytoskeleton supports vital processes in fungal cells, including hyphal growth and mitosis. Consequently, it is a target for fungicides, such as benomyl. The use of fluorescent fusion proteins to illuminate microtubules and microtubule-associated proteins has led to a break-through in our understanding of their dynamics and function in fungal cells. Here, we introduce fluorescent markers to visualize microtubules and accessory proteins in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein to α-tubulin (ZtTub2), to ZtPeb1, a homologue of the mammalian plus-end binding protein EB1, and to ZtGrc1, a component of the minus-end located γ-tubulin ring complex, involved in the nucleation of microtubules. In vivo observation confirms the localization and dynamic behaviour of all three markers. These marker proteins are useful tools for understanding the organization and importance of the microtubule cytoskeleton in Z. tritici.

KW - Ascomycota

KW - Cytoskeletal Proteins

KW - Genes, Reporter

KW - Luminescent Proteins

KW - Microtubules

KW - Optical Imaging

KW - Recombinant Fusion Proteins

KW - Staining and Labeling

U2 - 10.1016/j.fgb.2015.03.005

DO - 10.1016/j.fgb.2015.03.005

M3 - Journal article

C2 - 25857261

VL - 79

SP - 141

EP - 149

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

SN - 1087-1845

ER -

ID: 162342199