Fluorescent markers of the endocytic pathway in Zymoseptoria tritici
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Fluorescent markers of the endocytic pathway in Zymoseptoria tritici. / Kilaru, S; Schuster, M; Latz, M; Guo, M; Steinberg, G.
I: Fungal Genetics and Biology, Bind 79, 2015, s. 150-157.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Fluorescent markers of the endocytic pathway in Zymoseptoria tritici
AU - Kilaru, S
AU - Schuster, M
AU - Latz, M
AU - Guo, M
AU - Steinberg, G
N1 - Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Hyphal growth in filamentous fungi is supported by the uptake (endocytosis) and recycling of membranes and associated proteins at the growing tip. An increasing body of published evidence in various fungi demonstrates that this process is of essential importance for fungal growth and pathogenicity. Here, we introduce fluorescent markers to visualize the endocytic pathway in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein (eGFP) to the actin-binding protein fimbrin (ZtFim1), which is located in actin patches that are formed at the plasma membrane and are participating in endocytic uptake at the cell surface. In addition, we tagged early endosomes by eGFP-labelling a Rab5-homologue (ZtRab5) and late endosomes and vacuoles by expressing eGFP-Rab7 homologue (ZtRab7). Using fluorescent dyes and live cell imaging we confirmed the dynamic behavior and localization of these markers. This set of molecular tools enables an in-depth phenotypic analysis of Z. tritici mutant strains thereby supporting new strategies towards the goal of controlling wheat against Z. tritici.
AB - Hyphal growth in filamentous fungi is supported by the uptake (endocytosis) and recycling of membranes and associated proteins at the growing tip. An increasing body of published evidence in various fungi demonstrates that this process is of essential importance for fungal growth and pathogenicity. Here, we introduce fluorescent markers to visualize the endocytic pathway in the wheat pathogen Zymoseptoria tritici. We fused enhanced green-fluorescent protein (eGFP) to the actin-binding protein fimbrin (ZtFim1), which is located in actin patches that are formed at the plasma membrane and are participating in endocytic uptake at the cell surface. In addition, we tagged early endosomes by eGFP-labelling a Rab5-homologue (ZtRab5) and late endosomes and vacuoles by expressing eGFP-Rab7 homologue (ZtRab7). Using fluorescent dyes and live cell imaging we confirmed the dynamic behavior and localization of these markers. This set of molecular tools enables an in-depth phenotypic analysis of Z. tritici mutant strains thereby supporting new strategies towards the goal of controlling wheat against Z. tritici.
KW - Ascomycota
KW - Endocytosis
KW - Genes, Reporter
KW - Green Fluorescent Proteins
KW - Membrane Glycoproteins
KW - Microfilament Proteins
KW - Optical Imaging
KW - Recombinant Fusion Proteins
KW - Staining and Labeling
U2 - 10.1016/j.fgb.2015.03.019
DO - 10.1016/j.fgb.2015.03.019
M3 - Journal article
C2 - 26092801
VL - 79
SP - 150
EP - 157
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
ER -
ID: 162342095