TY - JOUR

T1 - Exposure of CuO nanoparticles and their metal counterpart leads to change in the gut microbiota and resistome of collembolans

AU - Ding, Jing

AU - Liu, Jin

AU - Chang, Xian Bo

AU - Zhu, Dong

AU - Lassen, Simon Bo

PY - 2020

Y1 - 2020

N2 - The widespread use of copper oxide nanoparticles (CuONPs) has dramatically increased their concentrations in soils and severely affected the health of soil organisms. The gut microbiota critically contributes to the metabolism and immune system of its host and is sensitive to environmental pollution. The toxic effect of CuONPs on the gut microbiota, especially in soil fauna, still needs further research. In the present study, a comprehensive toxicological test was performed to reveal the effects of CuONPs and their metal counterpart on the gut microbiota of soil collembolans using Illumina high throughput sequencing. Furthermore, the concomitant changes in the collembolans gut-associated antibiotic resistance genes (ARGs) and metabolism were investigated using high-throughput quantitative PCR and carbon and nitrogen stable isotope compositions. Both CuONPs and ionic copper (Cu) exposure disturbed the collembolan gut microbial community structure while only CuONPs reduced the gut microbial diversity. A total of 66 ARGs were detected in the collembolan guts, and CuONPs exposure induced a reduction in both diversity and abundance of ARGs. Additionally, CuONPs and ionic Cu exposure altered the C and N stable isotope compositions of the collembolans, indicating a change in their metabolism. Moreover, structural equation modeling indicated that 85.5% of the carbon stable isotope variations and 73.3% of the nitrogen stable isotope variations were explained by changes in Cu bioaccumulation and the gut microbiota. The results of the present study extend our knowledge regarding the comprehensive toxicity of metal oxide NPs on soil fauna. (C) 2020 Elsevier Ltd. All rights reserved.

AB - The widespread use of copper oxide nanoparticles (CuONPs) has dramatically increased their concentrations in soils and severely affected the health of soil organisms. The gut microbiota critically contributes to the metabolism and immune system of its host and is sensitive to environmental pollution. The toxic effect of CuONPs on the gut microbiota, especially in soil fauna, still needs further research. In the present study, a comprehensive toxicological test was performed to reveal the effects of CuONPs and their metal counterpart on the gut microbiota of soil collembolans using Illumina high throughput sequencing. Furthermore, the concomitant changes in the collembolans gut-associated antibiotic resistance genes (ARGs) and metabolism were investigated using high-throughput quantitative PCR and carbon and nitrogen stable isotope compositions. Both CuONPs and ionic copper (Cu) exposure disturbed the collembolan gut microbial community structure while only CuONPs reduced the gut microbial diversity. A total of 66 ARGs were detected in the collembolan guts, and CuONPs exposure induced a reduction in both diversity and abundance of ARGs. Additionally, CuONPs and ionic Cu exposure altered the C and N stable isotope compositions of the collembolans, indicating a change in their metabolism. Moreover, structural equation modeling indicated that 85.5% of the carbon stable isotope variations and 73.3% of the nitrogen stable isotope variations were explained by changes in Cu bioaccumulation and the gut microbiota. The results of the present study extend our knowledge regarding the comprehensive toxicity of metal oxide NPs on soil fauna. (C) 2020 Elsevier Ltd. All rights reserved.

KW - Nano-particles

KW - Gut microbiome

KW - Antibiotic resistance genes

KW - Isotope fractionation

KW - Soil fauna

KW - COPPER-OXIDE NANOPARTICLES

KW - RESISTANCE GENES

KW - SOIL

KW - FERTILIZATION

KW - DYSBIOSIS

KW - SELECTION

KW - BACTERIA

KW - TAXONOMY

KW - IMPACTS

KW - PLANTS

U2 - 10.1016/j.chemosphere.2020.127347

DO - 10.1016/j.chemosphere.2020.127347

M3 - Journal article

C2 - 32535433

VL - 258

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

M1 - 127347

ER -