Ectopic expression of OsJAZ6, which interacts with OsJAZ1, alters JA signaling and spikelet development in rice
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Ectopic expression of OsJAZ6, which interacts with OsJAZ1, alters JA signaling and spikelet development in rice. / Cao, Lichun; Tian, Jiaqi; Liu, Yilin; Chen, Xiaofei; Li, Siqi; Persson, Staffan; Lu, Dan; Chen, Mingjiao; Luo, Zhijing; Zhang, Dabing; Yuan, Zheng.
I: Plant Journal, Bind 108, Nr. 4, 2021, s. 1083-1096.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Ectopic expression of OsJAZ6, which interacts with OsJAZ1, alters JA signaling and spikelet development in rice
AU - Cao, Lichun
AU - Tian, Jiaqi
AU - Liu, Yilin
AU - Chen, Xiaofei
AU - Li, Siqi
AU - Persson, Staffan
AU - Lu, Dan
AU - Chen, Mingjiao
AU - Luo, Zhijing
AU - Zhang, Dabing
AU - Yuan, Zheng
N1 - Publisher Copyright: © 2021 Society for Experimental Biology and John Wiley & Sons Ltd.
PY - 2021
Y1 - 2021
N2 - Jasmonates (JAs) are key phytohormones that regulate plant responses and development. JASMONATE-ZIM DOMAIN (JAZ) proteins safeguard JA signaling by repressing JA-responsive gene expression in the absence of JA. However, the interaction and cooperative roles of JAZ repressors remain unclear during plant development. Here, we found that OsJAZ6 interacts with OsJAZ1 depending on a single amino acid in the so-called ZIM domain of OsJAZ6 in rice JA signaling transduction and JA-regulated rice spikelet development. In vivo protein distribution analysis revealed that the OsJAZ6 content is efficiently regulated during spikelet development, and biochemical and genetic evidence showed that OsJAZ6 is more sensitive to JA-mediated degradation than OsJAZ1. Through over- and mis-expression experiments, we further showed that the protein stability and levels of OsJAZ6 orchestrate the output of JA signaling during rice spikelet development. A possible mechanism, which outlines how OsJAZ repressors interact and function synergistically in specifying JA signaling output through degradation titration, is also discussed.
AB - Jasmonates (JAs) are key phytohormones that regulate plant responses and development. JASMONATE-ZIM DOMAIN (JAZ) proteins safeguard JA signaling by repressing JA-responsive gene expression in the absence of JA. However, the interaction and cooperative roles of JAZ repressors remain unclear during plant development. Here, we found that OsJAZ6 interacts with OsJAZ1 depending on a single amino acid in the so-called ZIM domain of OsJAZ6 in rice JA signaling transduction and JA-regulated rice spikelet development. In vivo protein distribution analysis revealed that the OsJAZ6 content is efficiently regulated during spikelet development, and biochemical and genetic evidence showed that OsJAZ6 is more sensitive to JA-mediated degradation than OsJAZ1. Through over- and mis-expression experiments, we further showed that the protein stability and levels of OsJAZ6 orchestrate the output of JA signaling during rice spikelet development. A possible mechanism, which outlines how OsJAZ repressors interact and function synergistically in specifying JA signaling output through degradation titration, is also discussed.
KW - degradation rate
KW - jasmonate
KW - OsJAZ repressor complex
KW - rice (Oryza sativa L.)
KW - spikelet
KW - stability
U2 - 10.1111/tpj.15496
DO - 10.1111/tpj.15496
M3 - Journal article
C2 - 34538009
AN - SCOPUS:85116402461
VL - 108
SP - 1083
EP - 1096
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 4
ER -
ID: 281983988