A putative O-methyltransferase from barley is induced by fungal pathogens and UV light
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A putative O-methyltransferase from barley is induced by fungal pathogens and UV light. / Gregersen, Per L.; Christensen, Anders B.; Sommer-Knudsen, Jens; Collinge, David B.
I: Plant Molecular Biology, Bind 26, Nr. 6, 01.12.1994, s. 1797-1806.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - A putative O-methyltransferase from barley is induced by fungal pathogens and UV light
AU - Gregersen, Per L.
AU - Christensen, Anders B.
AU - Sommer-Knudsen, Jens
AU - Collinge, David B.
PY - 1994/12/1
Y1 - 1994/12/1
N2 - A cDNA clone, pBH72-F1 (F1), was isolated from a cDNA library prepared from barley leaves 72 h after inoculation with Erysiphe graminis f.sp. hordei. The 1388 bp nucleotide sequence of pBH72-F1 contains an open reading frame encoding a 42.3 kDa polypeptide of 390 amino acids which shows sequence similarity to O-methyltransferases (OMTs) from different plant species; the highest identity (41%) was observed with a putative OMT expressed in roots of maize. A phylogenetic analysis shows that the barley and maize sequences are distinctly different from the ortho-diphenol-OMTs involved in lignin formation. A putative S-adenosyl-L-methionine-binding motif (KELVDDSITN) determined for a rabbit protein-carboxyl OMT is partially conserved in the encoded amino acid sequence. Genomic Southern blot hybridization shows that pBH72-F1 probably represents a single copy gene. The F1 clone corresponds to a gene transcript exhibiting a relatively late accumulation in mildew-infected barley leaves compared to other pathogen-induced transcripts, such as transcripts encoding PR proteins, a peroxidase, and transcripts homologous to a maize caffeic acid OMT. No transcript was detected in plants exhibiting papilla resistance at time points when resistance is thought to be manifested. The atypical transcript accumulation pattern for F1 was also observed after infection by other pathogens and after UV-light treatment.
AB - A cDNA clone, pBH72-F1 (F1), was isolated from a cDNA library prepared from barley leaves 72 h after inoculation with Erysiphe graminis f.sp. hordei. The 1388 bp nucleotide sequence of pBH72-F1 contains an open reading frame encoding a 42.3 kDa polypeptide of 390 amino acids which shows sequence similarity to O-methyltransferases (OMTs) from different plant species; the highest identity (41%) was observed with a putative OMT expressed in roots of maize. A phylogenetic analysis shows that the barley and maize sequences are distinctly different from the ortho-diphenol-OMTs involved in lignin formation. A putative S-adenosyl-L-methionine-binding motif (KELVDDSITN) determined for a rabbit protein-carboxyl OMT is partially conserved in the encoded amino acid sequence. Genomic Southern blot hybridization shows that pBH72-F1 probably represents a single copy gene. The F1 clone corresponds to a gene transcript exhibiting a relatively late accumulation in mildew-infected barley leaves compared to other pathogen-induced transcripts, such as transcripts encoding PR proteins, a peroxidase, and transcripts homologous to a maize caffeic acid OMT. No transcript was detected in plants exhibiting papilla resistance at time points when resistance is thought to be manifested. The atypical transcript accumulation pattern for F1 was also observed after infection by other pathogens and after UV-light treatment.
KW - defence response
KW - Erysiphe graminis
KW - Hordeum vulgare
KW - O-methyltransferase
KW - phenylpropanoid metabolism
UR - http://www.scopus.com/inward/record.url?scp=0028675453&partnerID=8YFLogxK
U2 - 10.1007/BF00019493
DO - 10.1007/BF00019493
M3 - Journal article
C2 - 7858218
AN - SCOPUS:0028675453
VL - 26
SP - 1797
EP - 1806
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 6
ER -
ID: 201509634