Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining

Department of Plant and Environmental Sciences

Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining. / Baum, Julia F.; Uzun, Huriye D.; Pomorski, Thomas Günther.

In: Bio-protocol, Vol. 13, No. 14, e4754, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Baum, JF, Uzun, HD & Pomorski, TG 2023, 'Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining', Bio-protocol, vol. 13, no. 14, e4754. https://doi.org/10.21769/BioProtoc.4754

APA

Baum, J. F., Uzun, H. D., & Pomorski, T. G. (2023). Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining. Bio-protocol, 13(14), [e4754]. https://doi.org/10.21769/BioProtoc.4754

Vancouver

Baum JF, Uzun HD, Pomorski TG. Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining. Bio-protocol. 2023;13(14). e4754. https://doi.org/10.21769/BioProtoc.4754

Author

Baum, Julia F. ; Uzun, Huriye D. ; Pomorski, Thomas Günther. / Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining. In: Bio-protocol. 2023 ; Vol. 13, No. 14.

Bibtex

@article{0da86dd68d9342e8bbdbd4af3c5afe05,

title = "Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining",

abstract = "Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion. In this protocol, we describe the use of fluorescently labeled annexin V to detect loss of lipid asymmetry in the plasma membrane of adherent living cells by fluorescence microscopy. The approach provides a simple, sensitive, and reproducible method to detect changes in lipid asymmetry but is limited by low sample throughput. The protocol can also be adapted to other fluorescently labeled lipid-binding proteins or peptide probes. To validate the lipid binding properties of such probes, we additionally describe here the preparation and use of giant unilamellar vesicles as simple model membrane systems that have a size comparable to cells.",

keywords = "Confocal microscopy, Giant vesicle, Lipid asymmetry, Lipid-binding protein, Mammalian cells, Plasma membrane",

author = "Baum, {Julia F.} and Uzun, {Huriye D.} and Pomorski, {Thomas G{\"u}nther}",

note = "Publisher Copyright: {\textcopyright} 2023 The Authors; exclusive licensee Bio-protocol LLC.",

year = "2023",

doi = "10.21769/BioProtoc.4754",

language = "English",

volume = "13",

journal = "Bio-protocol",

issn = "2331-8325",

publisher = "bio-protocol",

number = "14",

}

RIS

TY - JOUR

T1 - Visualizing Loss of Plasma Membrane Lipid Asymmetry Using Annexin V Staining

AU - Baum, Julia F.

AU - Uzun, Huriye D.

AU - Pomorski, Thomas Günther

PY - 2023

Y1 - 2023

N2 - Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion. In this protocol, we describe the use of fluorescently labeled annexin V to detect loss of lipid asymmetry in the plasma membrane of adherent living cells by fluorescence microscopy. The approach provides a simple, sensitive, and reproducible method to detect changes in lipid asymmetry but is limited by low sample throughput. The protocol can also be adapted to other fluorescently labeled lipid-binding proteins or peptide probes. To validate the lipid binding properties of such probes, we additionally describe here the preparation and use of giant unilamellar vesicles as simple model membrane systems that have a size comparable to cells.

AB - Loss of plasma membrane lipid asymmetry contributes to many cellular functions and responses, including apoptosis, blood coagulation, and cell fusion. In this protocol, we describe the use of fluorescently labeled annexin V to detect loss of lipid asymmetry in the plasma membrane of adherent living cells by fluorescence microscopy. The approach provides a simple, sensitive, and reproducible method to detect changes in lipid asymmetry but is limited by low sample throughput. The protocol can also be adapted to other fluorescently labeled lipid-binding proteins or peptide probes. To validate the lipid binding properties of such probes, we additionally describe here the preparation and use of giant unilamellar vesicles as simple model membrane systems that have a size comparable to cells.

KW - Confocal microscopy

KW - Giant vesicle

KW - Lipid asymmetry

KW - Lipid-binding protein

KW - Mammalian cells

KW - Plasma membrane

U2 - 10.21769/BioProtoc.4754

DO - 10.21769/BioProtoc.4754

M3 - Journal article

C2 - 37497452

AN - SCOPUS:85166533756

VL - 13

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 14

M1 - e4754

ER -

ID: 364546282