VC1 catalyses a key step in the biosynthesis of vicine in faba bean

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

VC1 catalyses a key step in the biosynthesis of vicine in faba bean. / Björnsdotter, Emilie; Nadzieja, Marcin; Chang, Wei; Escobar-Herrera, Leandro; Mancinotti, Davide; Angra, Deepti; Xia, Xinxing; Tacke, Rebecca; Khazaei, Hamid; Crocoll, Christoph; Vandenberg, Albert; Link, Wolfgang; Stoddard, Frederick L.; O'Sullivan, Donal M.; Stougaard, Jens; Schulman, Alan H.; Andersen, Stig U.; Geu-Flores, Fernando.

In: Nature Plants, Vol. 7, 2021, p. 923–931.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Björnsdotter, E, Nadzieja, M, Chang, W, Escobar-Herrera, L, Mancinotti, D, Angra, D, Xia, X, Tacke, R, Khazaei, H, Crocoll, C, Vandenberg, A, Link, W, Stoddard, FL, O'Sullivan, DM, Stougaard, J, Schulman, AH, Andersen, SU & Geu-Flores, F 2021, 'VC1 catalyses a key step in the biosynthesis of vicine in faba bean', Nature Plants, vol. 7, pp. 923–931. https://doi.org/10.1038/s41477-021-00950-w

APA

Björnsdotter, E., Nadzieja, M., Chang, W., Escobar-Herrera, L., Mancinotti, D., Angra, D., Xia, X., Tacke, R., Khazaei, H., Crocoll, C., Vandenberg, A., Link, W., Stoddard, F. L., O'Sullivan, D. M., Stougaard, J., Schulman, A. H., Andersen, S. U., & Geu-Flores, F. (2021). VC1 catalyses a key step in the biosynthesis of vicine in faba bean. Nature Plants, 7, 923–931. https://doi.org/10.1038/s41477-021-00950-w

Vancouver

Björnsdotter E, Nadzieja M, Chang W, Escobar-Herrera L, Mancinotti D, Angra D et al. VC1 catalyses a key step in the biosynthesis of vicine in faba bean. Nature Plants. 2021;7:923–931. https://doi.org/10.1038/s41477-021-00950-w

Author

Björnsdotter, Emilie ; Nadzieja, Marcin ; Chang, Wei ; Escobar-Herrera, Leandro ; Mancinotti, Davide ; Angra, Deepti ; Xia, Xinxing ; Tacke, Rebecca ; Khazaei, Hamid ; Crocoll, Christoph ; Vandenberg, Albert ; Link, Wolfgang ; Stoddard, Frederick L. ; O'Sullivan, Donal M. ; Stougaard, Jens ; Schulman, Alan H. ; Andersen, Stig U. ; Geu-Flores, Fernando. / VC1 catalyses a key step in the biosynthesis of vicine in faba bean. In: Nature Plants. 2021 ; Vol. 7. pp. 923–931.

Bibtex

@article{a47c80e12c6c4e7b904bbf3a1399dcce,
title = "VC1 catalyses a key step in the biosynthesis of vicine in faba bean",
abstract = "Faba bean (Vicia faba L.) is a widely adapted and high-yielding legume cultivated for its protein-rich seeds(1). However, the seeds accumulate the pyrimidine glucosides vicine and convicine, which can cause haemolytic anaemia (favism) in 400 million genetically predisposed individuals(2). Here, we use gene-to-metabolite correlations, gene mapping and genetic complementation to identify VC1 as a key enzyme in vicine and convicine biosynthesis. We demonstrate that VC1 has GTP cyclohydrolase II activity and that the purine GTP is a precursor of both vicine and convicine. Finally, we show that cultivars with low vicine and convicine levels carry an inactivating insertion in the coding sequence of VC1. Our results reveal an unexpected, purine rather than pyrimidine, biosynthetic origin for vicine and convicine and pave the way for the development of faba bean cultivars that are free of these anti-nutrients.",
keywords = "GTP CYCLOHYDROLASE-II, AGROBACTERIUM-RHIZOGENES, CONVICINE",
author = "Emilie Bj{\"o}rnsdotter and Marcin Nadzieja and Wei Chang and Leandro Escobar-Herrera and Davide Mancinotti and Deepti Angra and Xinxing Xia and Rebecca Tacke and Hamid Khazaei and Christoph Crocoll and Albert Vandenberg and Wolfgang Link and Stoddard, {Frederick L.} and O'Sullivan, {Donal M.} and Jens Stougaard and Schulman, {Alan H.} and Andersen, {Stig U.} and Fernando Geu-Flores",
year = "2021",
doi = "10.1038/s41477-021-00950-w",
language = "English",
volume = "7",
pages = "923–931",
journal = "Nature Plants",
issn = "2055-026X",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - VC1 catalyses a key step in the biosynthesis of vicine in faba bean

AU - Björnsdotter, Emilie

AU - Nadzieja, Marcin

AU - Chang, Wei

AU - Escobar-Herrera, Leandro

AU - Mancinotti, Davide

AU - Angra, Deepti

AU - Xia, Xinxing

AU - Tacke, Rebecca

AU - Khazaei, Hamid

AU - Crocoll, Christoph

AU - Vandenberg, Albert

AU - Link, Wolfgang

AU - Stoddard, Frederick L.

AU - O'Sullivan, Donal M.

AU - Stougaard, Jens

AU - Schulman, Alan H.

AU - Andersen, Stig U.

AU - Geu-Flores, Fernando

PY - 2021

Y1 - 2021

N2 - Faba bean (Vicia faba L.) is a widely adapted and high-yielding legume cultivated for its protein-rich seeds(1). However, the seeds accumulate the pyrimidine glucosides vicine and convicine, which can cause haemolytic anaemia (favism) in 400 million genetically predisposed individuals(2). Here, we use gene-to-metabolite correlations, gene mapping and genetic complementation to identify VC1 as a key enzyme in vicine and convicine biosynthesis. We demonstrate that VC1 has GTP cyclohydrolase II activity and that the purine GTP is a precursor of both vicine and convicine. Finally, we show that cultivars with low vicine and convicine levels carry an inactivating insertion in the coding sequence of VC1. Our results reveal an unexpected, purine rather than pyrimidine, biosynthetic origin for vicine and convicine and pave the way for the development of faba bean cultivars that are free of these anti-nutrients.

AB - Faba bean (Vicia faba L.) is a widely adapted and high-yielding legume cultivated for its protein-rich seeds(1). However, the seeds accumulate the pyrimidine glucosides vicine and convicine, which can cause haemolytic anaemia (favism) in 400 million genetically predisposed individuals(2). Here, we use gene-to-metabolite correlations, gene mapping and genetic complementation to identify VC1 as a key enzyme in vicine and convicine biosynthesis. We demonstrate that VC1 has GTP cyclohydrolase II activity and that the purine GTP is a precursor of both vicine and convicine. Finally, we show that cultivars with low vicine and convicine levels carry an inactivating insertion in the coding sequence of VC1. Our results reveal an unexpected, purine rather than pyrimidine, biosynthetic origin for vicine and convicine and pave the way for the development of faba bean cultivars that are free of these anti-nutrients.

KW - GTP CYCLOHYDROLASE-II

KW - AGROBACTERIUM-RHIZOGENES

KW - CONVICINE

U2 - 10.1038/s41477-021-00950-w

DO - 10.1038/s41477-021-00950-w

M3 - Journal article

C2 - 34226693

VL - 7

SP - 923

EP - 931

JO - Nature Plants

JF - Nature Plants

SN - 2055-026X

ER -

ID: 275380579