The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth. / Davis, Jeffrey A.; Pares, Randall B.; Bernstein, Tilde; McDowell, Stephen C.; Brown, Elizabeth; Stubrich, Jason; Rosenberg, Alexa; Cahoon, Edgar B.; Cahoon, Rebecca E.; Poulsen, Lisbeth R.; Palmgren, Michael; López-Marqués, Rosa L.; Harper, Jeffrey F.
In: Plant Physiology, Vol. 182, No. 4, 2020, p. 2111-2125.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The lipid flippases ALA4 and ALA5 play critical roles in cell expansion and plant growth
AU - Davis, Jeffrey A.
AU - Pares, Randall B.
AU - Bernstein, Tilde
AU - McDowell, Stephen C.
AU - Brown, Elizabeth
AU - Stubrich, Jason
AU - Rosenberg, Alexa
AU - Cahoon, Edgar B.
AU - Cahoon, Rebecca E.
AU - Poulsen, Lisbeth R.
AU - Palmgren, Michael
AU - López-Marqués, Rosa L.
AU - Harper, Jeffrey F.
PY - 2020
Y1 - 2020
N2 - Aminophospholipid ATPases (ALAs) are lipid flippases involved in transporting specific lipids across membrane bilayers. Arabidopsis (Arabidopsis thaliana) contains 12 ALAs in five phylogenetic clusters, including four in cluster 3 (ALA4-ALA7). ALA4/5 and ALA6/7, are expressed primarily in vegetative tissues and pollen, respectively. Previously, a double knockout of ALA6/7 was shown to result in pollen fertility defects. Here we show that a double knockout of ALA4/5 results in dwarfism, characterized by reduced growth in rosettes (6.5-fold), roots (4.3-fold), bolts (4.5-fold), and hypocotyls (2-fold). Reduced cell size was observed for multiple vegetative cell types, suggesting a role for ALA4/5 in cellular expansion. Members of the third ALA cluster are at least partially interchangeable, as transgenes expressing ALA6 in vegetative tissues partially rescued ala4/5 mutant phenotypes, and expression of ALA4 transgenes in pollen fully rescued ala6/7 mutant fertility defects. ALA4-GFP displayed plasma membrane and endomembrane localization patterns when imaged in both guard cells and pollen. Lipid profiling revealed ala4/5 rosettes had perturbations in glycerolipid and sphingolipid content. Assays in yeast revealed that ALA5 can flip a variety of glycerolipids and the sphingolipid sphingomyelin across membranes. These results support a model whereby the flippase activity of ALA4 and ALA5 impacts the homeostasis of both glycerolipids and sphingolipids and is important for cellular expansion during vegetative growth.
AB - Aminophospholipid ATPases (ALAs) are lipid flippases involved in transporting specific lipids across membrane bilayers. Arabidopsis (Arabidopsis thaliana) contains 12 ALAs in five phylogenetic clusters, including four in cluster 3 (ALA4-ALA7). ALA4/5 and ALA6/7, are expressed primarily in vegetative tissues and pollen, respectively. Previously, a double knockout of ALA6/7 was shown to result in pollen fertility defects. Here we show that a double knockout of ALA4/5 results in dwarfism, characterized by reduced growth in rosettes (6.5-fold), roots (4.3-fold), bolts (4.5-fold), and hypocotyls (2-fold). Reduced cell size was observed for multiple vegetative cell types, suggesting a role for ALA4/5 in cellular expansion. Members of the third ALA cluster are at least partially interchangeable, as transgenes expressing ALA6 in vegetative tissues partially rescued ala4/5 mutant phenotypes, and expression of ALA4 transgenes in pollen fully rescued ala6/7 mutant fertility defects. ALA4-GFP displayed plasma membrane and endomembrane localization patterns when imaged in both guard cells and pollen. Lipid profiling revealed ala4/5 rosettes had perturbations in glycerolipid and sphingolipid content. Assays in yeast revealed that ALA5 can flip a variety of glycerolipids and the sphingolipid sphingomyelin across membranes. These results support a model whereby the flippase activity of ALA4 and ALA5 impacts the homeostasis of both glycerolipids and sphingolipids and is important for cellular expansion during vegetative growth.
U2 - 10.1104/PP.19.01332
DO - 10.1104/PP.19.01332
M3 - Journal article
C2 - 32051180
AN - SCOPUS:85086172178
VL - 182
SP - 2111
EP - 2125
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 4
ER -
ID: 245000107