The causal mutation leading to sweetness in modern white lupin cultivars

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The causal mutation leading to sweetness in modern white lupin cultivars. / Mancinotti, Davide; Czepiel, Katarzyna; Taylor, Jemma L.; Galehshahi, Hajar Golshadi; Møller, Lillian A.; Jensen, Mikkel K.; Motawia, Mohammed Saddik; Hufnagel, Bárbara; Soriano, Alexandre; Yeheyis, Likawent; Kjaerulff, Louise; Péret, Benjamin; Staerk, Dan; Wendt, Toni; Nelson, Matthew N.; Kroc, Magdalena; Geu-Flores, Fernando.

In: Science Advances, Vol. 9, No. 31, eadg8866, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mancinotti, D, Czepiel, K, Taylor, JL, Galehshahi, HG, Møller, LA, Jensen, MK, Motawia, MS, Hufnagel, B, Soriano, A, Yeheyis, L, Kjaerulff, L, Péret, B, Staerk, D, Wendt, T, Nelson, MN, Kroc, M & Geu-Flores, F 2023, 'The causal mutation leading to sweetness in modern white lupin cultivars', Science Advances, vol. 9, no. 31, eadg8866. https://doi.org/10.1126/sciadv.adg8866

APA

Mancinotti, D., Czepiel, K., Taylor, J. L., Galehshahi, H. G., Møller, L. A., Jensen, M. K., Motawia, M. S., Hufnagel, B., Soriano, A., Yeheyis, L., Kjaerulff, L., Péret, B., Staerk, D., Wendt, T., Nelson, M. N., Kroc, M., & Geu-Flores, F. (2023). The causal mutation leading to sweetness in modern white lupin cultivars. Science Advances, 9(31), [eadg8866]. https://doi.org/10.1126/sciadv.adg8866

Vancouver

Mancinotti D, Czepiel K, Taylor JL, Galehshahi HG, Møller LA, Jensen MK et al. The causal mutation leading to sweetness in modern white lupin cultivars. Science Advances. 2023;9(31). eadg8866. https://doi.org/10.1126/sciadv.adg8866

Author

Mancinotti, Davide ; Czepiel, Katarzyna ; Taylor, Jemma L. ; Galehshahi, Hajar Golshadi ; Møller, Lillian A. ; Jensen, Mikkel K. ; Motawia, Mohammed Saddik ; Hufnagel, Bárbara ; Soriano, Alexandre ; Yeheyis, Likawent ; Kjaerulff, Louise ; Péret, Benjamin ; Staerk, Dan ; Wendt, Toni ; Nelson, Matthew N. ; Kroc, Magdalena ; Geu-Flores, Fernando. / The causal mutation leading to sweetness in modern white lupin cultivars. In: Science Advances. 2023 ; Vol. 9, No. 31.

Bibtex

@article{8981f443b052446f8a842da87f530d22,
title = "The causal mutation leading to sweetness in modern white lupin cultivars",
abstract = "Lupins are high-protein crops that are rapidly gaining interest as hardy alternatives to soybean; however, they accumulate antinutritional alkaloids of the quinolizidine type (QAs). Lupin domestication was enabled by the discovery of genetic loci conferring low QA levels (sweetness), but the precise identity of the underlying genes remains uncertain. We show that pauper, the most common sweet locus in white lupin, encodes an acetyltransferase (AT) unexpectedly involved in the early QA pathway. In pauper plants, a single-nucleotide polymorphism (SNP) strongly impairs AT activity, causing pathway blockage. We corroborate our hypothesis by replicating the pauper chemotype in narrow-leafed lupin via mutagenesis. Our work adds a new dimension to QA biosynthesis and establishes the identity of a lupin sweet gene for the first time, thus facilitating lupin breeding and enabling domestication of other QA-containing legumes.",
author = "Davide Mancinotti and Katarzyna Czepiel and Taylor, {Jemma L.} and Galehshahi, {Hajar Golshadi} and M{\o}ller, {Lillian A.} and Jensen, {Mikkel K.} and Motawia, {Mohammed Saddik} and B{\'a}rbara Hufnagel and Alexandre Soriano and Likawent Yeheyis and Louise Kjaerulff and Benjamin P{\'e}ret and Dan Staerk and Toni Wendt and Nelson, {Matthew N.} and Magdalena Kroc and Fernando Geu-Flores",
note = "Publisher Copyright: Copyright {\textcopyright} 2023 The Authors, some rights reserved.",
year = "2023",
doi = "10.1126/sciadv.adg8866",
language = "English",
volume = "9",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "31",

}

RIS

TY - JOUR

T1 - The causal mutation leading to sweetness in modern white lupin cultivars

AU - Mancinotti, Davide

AU - Czepiel, Katarzyna

AU - Taylor, Jemma L.

AU - Galehshahi, Hajar Golshadi

AU - Møller, Lillian A.

AU - Jensen, Mikkel K.

AU - Motawia, Mohammed Saddik

AU - Hufnagel, Bárbara

AU - Soriano, Alexandre

AU - Yeheyis, Likawent

AU - Kjaerulff, Louise

AU - Péret, Benjamin

AU - Staerk, Dan

AU - Wendt, Toni

AU - Nelson, Matthew N.

AU - Kroc, Magdalena

AU - Geu-Flores, Fernando

N1 - Publisher Copyright: Copyright © 2023 The Authors, some rights reserved.

PY - 2023

Y1 - 2023

N2 - Lupins are high-protein crops that are rapidly gaining interest as hardy alternatives to soybean; however, they accumulate antinutritional alkaloids of the quinolizidine type (QAs). Lupin domestication was enabled by the discovery of genetic loci conferring low QA levels (sweetness), but the precise identity of the underlying genes remains uncertain. We show that pauper, the most common sweet locus in white lupin, encodes an acetyltransferase (AT) unexpectedly involved in the early QA pathway. In pauper plants, a single-nucleotide polymorphism (SNP) strongly impairs AT activity, causing pathway blockage. We corroborate our hypothesis by replicating the pauper chemotype in narrow-leafed lupin via mutagenesis. Our work adds a new dimension to QA biosynthesis and establishes the identity of a lupin sweet gene for the first time, thus facilitating lupin breeding and enabling domestication of other QA-containing legumes.

AB - Lupins are high-protein crops that are rapidly gaining interest as hardy alternatives to soybean; however, they accumulate antinutritional alkaloids of the quinolizidine type (QAs). Lupin domestication was enabled by the discovery of genetic loci conferring low QA levels (sweetness), but the precise identity of the underlying genes remains uncertain. We show that pauper, the most common sweet locus in white lupin, encodes an acetyltransferase (AT) unexpectedly involved in the early QA pathway. In pauper plants, a single-nucleotide polymorphism (SNP) strongly impairs AT activity, causing pathway blockage. We corroborate our hypothesis by replicating the pauper chemotype in narrow-leafed lupin via mutagenesis. Our work adds a new dimension to QA biosynthesis and establishes the identity of a lupin sweet gene for the first time, thus facilitating lupin breeding and enabling domestication of other QA-containing legumes.

U2 - 10.1126/sciadv.adg8866

DO - 10.1126/sciadv.adg8866

M3 - Journal article

C2 - 37540741

AN - SCOPUS:85166599048

VL - 9

JO - Science advances

JF - Science advances

SN - 2375-2548

IS - 31

M1 - eadg8866

ER -

ID: 368339104