Latent manganese deficiency increases transpiration in barley (Hordeum vulgare)
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Latent manganese deficiency increases transpiration in barley (Hordeum vulgare). / Hebbern, Christopher Alan; Laursen, Kristian Holst; Ladegaard, Anne Hald; Schmidt, Sidsel Birkelund; Pedas, Pai Rosager; Bruhn, Dan; Schjørring, Jan Kofod; Wulfsohn, Dvora-Laio; Husted, Søren.
In: Physiologia Plantarum : An International Journal for Plant Biology, Vol. 135, No. 3, 2009, p. 307-316.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Latent manganese deficiency increases transpiration in barley (Hordeum vulgare)
AU - Hebbern, Christopher Alan
AU - Laursen, Kristian Holst
AU - Ladegaard, Anne Hald
AU - Schmidt, Sidsel Birkelund
AU - Pedas, Pai Rosager
AU - Bruhn, Dan
AU - Schjørring, Jan Kofod
AU - Wulfsohn, Dvora-Laio
AU - Husted, Søren
PY - 2009
Y1 - 2009
N2 - To investigate if latent manganese (Mn) deficiency leads to increased transpiration, barley plants were grown for 10 weeks in hydroponics with daily additions of Mn in the low nM range. The Mn-starved plants did not exhibit visual leaf symptoms of Mn deficiency, but Chl a fluorescence measurements revealed that the quantum yield efficiency of PSII (Fv/Fm) was reduced from 0.83 in Mn-sufficient control plants to below 0.5 in Mn-starved plants. Leaf Mn concentrations declined from 30 to 7 µg Mn g-1 dry weight in control and Mn-starved plants, respectively. Mn-starved plants had up to four-fold higher transpiration than control plants. Stomatal closure and opening upon light/dark transitions took place at the same rate in both Mn treatments, but the nocturnal leaf conductance for water vapour was still twice as high in Mn-starved plants compared with the control. The observed increase in transpiration was substantiated by 13C-isotope discrimination analysis and gravimetric measurement of the water consumption, showing significantly lower water use efficiency in Mn-starved plants. The extractable wax content of leaves of Mn-starved plants was approximately 40% lower than that in control plants, and it is concluded that the increased leaf conductance and higher transpirational water loss are correlated with a reduction in the epicuticular wax layer under Mn deficiency.
AB - To investigate if latent manganese (Mn) deficiency leads to increased transpiration, barley plants were grown for 10 weeks in hydroponics with daily additions of Mn in the low nM range. The Mn-starved plants did not exhibit visual leaf symptoms of Mn deficiency, but Chl a fluorescence measurements revealed that the quantum yield efficiency of PSII (Fv/Fm) was reduced from 0.83 in Mn-sufficient control plants to below 0.5 in Mn-starved plants. Leaf Mn concentrations declined from 30 to 7 µg Mn g-1 dry weight in control and Mn-starved plants, respectively. Mn-starved plants had up to four-fold higher transpiration than control plants. Stomatal closure and opening upon light/dark transitions took place at the same rate in both Mn treatments, but the nocturnal leaf conductance for water vapour was still twice as high in Mn-starved plants compared with the control. The observed increase in transpiration was substantiated by 13C-isotope discrimination analysis and gravimetric measurement of the water consumption, showing significantly lower water use efficiency in Mn-starved plants. The extractable wax content of leaves of Mn-starved plants was approximately 40% lower than that in control plants, and it is concluded that the increased leaf conductance and higher transpirational water loss are correlated with a reduction in the epicuticular wax layer under Mn deficiency.
U2 - 10.1111/j.1399-3054.2008.01188.x
DO - 10.1111/j.1399-3054.2008.01188.x
M3 - Journal article
C2 - 19140891
VL - 135
SP - 307
EP - 316
JO - Physiologia Plantarum
JF - Physiologia Plantarum
SN - 0031-9317
IS - 3
ER -
ID: 11008110