Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis

Research output: Contribution to journal › Letter › Research › peer-review

Standard

Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis. / Justesen, Bo Højen; Laursen, Tomas; Weber, Gerhard ; Fuglsang, Anja Thoe; Møller, Birger Lindberg; Günther-Pomorski, Thomas.

In: Analytical Chemistry, Vol. 85, No. 7, 2013, p. 3497-3500.

Research output: Contribution to journal › Letter › Research › peer-review

Harvard

Justesen, BH, Laursen, T, Weber, G, Fuglsang, AT, Møller, BL & Günther-Pomorski, T 2013, 'Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis', Analytical Chemistry, vol. 85, no. 7, pp. 3497-3500. https://doi.org/10.1021/ac4000915

APA

Justesen, B. H., Laursen, T., Weber, G., Fuglsang, A. T., Møller, B. L., & Günther-Pomorski, T. (2013). Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis. Analytical Chemistry, 85(7), 3497-3500. https://doi.org/10.1021/ac4000915

Vancouver

Justesen BH, Laursen T, Weber G, Fuglsang AT, Møller BL, Günther-Pomorski T. Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis. Analytical Chemistry. 2013;85(7):3497-3500. https://doi.org/10.1021/ac4000915

Author

Justesen, Bo Højen ; Laursen, Tomas ; Weber, Gerhard ; Fuglsang, Anja Thoe ; Møller, Birger Lindberg ; Günther-Pomorski, Thomas. / Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis. In: Analytical Chemistry. 2013 ; Vol. 85, No. 7. pp. 3497-3500.

Bibtex

@article{ff05081472854940862a78c2a031de66,
title = "Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis",
abstract = "Free flow electrophoresis is used for rapid and high-recovery isolation of homogeneous preparations of functionally active membrane proteins inserted into nanodiscs. The approach enables isolation of integral and membrane anchored proteins and is also applicable following introduction of, e.g., fluorescent tags. Preparative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse nanodisc preparations ideal for structural and functional characterization using biophysical methods.",
author = "Justesen, {Bo H{\o}jen} and Tomas Laursen and Gerhard Weber and Fuglsang, {Anja Thoe} and M{\o}ller, {Birger Lindberg} and Thomas G{\"u}nther-Pomorski",
year = "2013",
doi = "10.1021/ac4000915",
language = "English",
volume = "85",
pages = "3497--3500",
journal = "Industrial And Engineering Chemistry Analytical Edition",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "7",

}

RIS

TY - JOUR

T1 - Isolation of monodisperse nanodisc-reconstituted membrane proteins using free flow electrophoresis

AU - Justesen, Bo Højen

AU - Laursen, Tomas

AU - Weber, Gerhard

AU - Fuglsang, Anja Thoe

AU - Møller, Birger Lindberg

AU - Günther-Pomorski, Thomas

PY - 2013

Y1 - 2013

N2 - Free flow electrophoresis is used for rapid and high-recovery isolation of homogeneous preparations of functionally active membrane proteins inserted into nanodiscs. The approach enables isolation of integral and membrane anchored proteins and is also applicable following introduction of, e.g., fluorescent tags. Preparative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse nanodisc preparations ideal for structural and functional characterization using biophysical methods.

AB - Free flow electrophoresis is used for rapid and high-recovery isolation of homogeneous preparations of functionally active membrane proteins inserted into nanodiscs. The approach enables isolation of integral and membrane anchored proteins and is also applicable following introduction of, e.g., fluorescent tags. Preparative separation of membrane protein loaded nanodiscs from empty nanodiscs and protein aggregates results in monodisperse nanodisc preparations ideal for structural and functional characterization using biophysical methods.

UR - http://www.scopus.com/inward/record.url?scp=84875771594&partnerID=8YFLogxK

U2 - 10.1021/ac4000915

DO - 10.1021/ac4000915

M3 - Letter

AN - SCOPUS:84875771594

VL - 85

SP - 3497

EP - 3500

JO - Industrial And Engineering Chemistry Analytical Edition

JF - Industrial And Engineering Chemistry Analytical Edition

SN - 0003-2700

IS - 7

ER -

ID: 45559322