Invited Mini-Review Research Topic: Utilization of Protoplasts to Facilitate Gene Editing in Plants: Schemes for In Vitro Shoot Regeneration From Tissues and Protoplasts of Potato and Rapeseed: Implications of Bioengineering Such as Gene Editing of Broad-Leaved Plants
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Invited Mini-Review Research Topic: Utilization of Protoplasts to Facilitate Gene Editing in Plants : Schemes for In Vitro Shoot Regeneration From Tissues and Protoplasts of Potato and Rapeseed: Implications of Bioengineering Such as Gene Editing of Broad-Leaved Plants. / Andreasson, Erik; Kieu, Nam Phuong; Zahid, Muhammad Awais; Carlsen, Frida Meijer; Marit, Lenman; Sandgrind, Sjur; Petersen, Bent Larsen; Zhu, Li Hua.
In: Frontiers in Genome Editing, Vol. 4, 780004, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Invited Mini-Review Research Topic: Utilization of Protoplasts to Facilitate Gene Editing in Plants
T2 - Schemes for In Vitro Shoot Regeneration From Tissues and Protoplasts of Potato and Rapeseed: Implications of Bioengineering Such as Gene Editing of Broad-Leaved Plants
AU - Andreasson, Erik
AU - Kieu, Nam Phuong
AU - Zahid, Muhammad Awais
AU - Carlsen, Frida Meijer
AU - Marit, Lenman
AU - Sandgrind, Sjur
AU - Petersen, Bent Larsen
AU - Zhu, Li Hua
N1 - Publisher Copyright: Copyright © 2022 Andreasson, Kieu, Zahid, Carlsen, Marit, Sandgrind, Petersen and Zhu.
PY - 2022
Y1 - 2022
N2 - Schemes for efficient regenerationand recovery of shoots from in vitro tissues or single cells, such as protoplasts, are only available for limited numbers of plant species and genotypes and are crucial for establishing gene editing tools on a broader scale in agriculture and plant biology. Growth conditions, including hormone and nutrient composition as well as light regimes in key steps of known regeneration protocols, display significant variations, even between the genotypes within the same species, e.g., potato (Solanum tuberosum). As fresh plant material is a prerequisite for successful shoot regeneration, the plant material often needs to be refreshed for optimizing the growth and physiological state prior to genetic transformation. Utilization of protoplasts has become a more important approach for obtaining transgene-free edited plants by genome editing, CRISPR/Cas9. In this approach, callus formation from protoplasts is induced by one set of hormones, followed by organogenesis, i.e., shoot formation, which is induced by a second set of hormones. The requirements on culture conditions at these key steps vary considerably between the species and genotypes, which often require quantitative adjustments of medium compositions. In this mini-review, we outline the protocols and notes for clonal regeneration and cultivation from single cells, particularly protoplasts in potato and rapeseed. We focus mainly on different hormone treatment schemes and highlight the importance of medium compositions, e.g., sugar, nutrient, and light regimes as well as culture durations at the key regeneration steps. We believe that this review would provide important information and hints for establishing efficient regeneration strategies from other closely related and broad-leaved plant species in general.
AB - Schemes for efficient regenerationand recovery of shoots from in vitro tissues or single cells, such as protoplasts, are only available for limited numbers of plant species and genotypes and are crucial for establishing gene editing tools on a broader scale in agriculture and plant biology. Growth conditions, including hormone and nutrient composition as well as light regimes in key steps of known regeneration protocols, display significant variations, even between the genotypes within the same species, e.g., potato (Solanum tuberosum). As fresh plant material is a prerequisite for successful shoot regeneration, the plant material often needs to be refreshed for optimizing the growth and physiological state prior to genetic transformation. Utilization of protoplasts has become a more important approach for obtaining transgene-free edited plants by genome editing, CRISPR/Cas9. In this approach, callus formation from protoplasts is induced by one set of hormones, followed by organogenesis, i.e., shoot formation, which is induced by a second set of hormones. The requirements on culture conditions at these key steps vary considerably between the species and genotypes, which often require quantitative adjustments of medium compositions. In this mini-review, we outline the protocols and notes for clonal regeneration and cultivation from single cells, particularly protoplasts in potato and rapeseed. We focus mainly on different hormone treatment schemes and highlight the importance of medium compositions, e.g., sugar, nutrient, and light regimes as well as culture durations at the key regeneration steps. We believe that this review would provide important information and hints for establishing efficient regeneration strategies from other closely related and broad-leaved plant species in general.
KW - Brassica napus
KW - gene editing
KW - potato
KW - protoplast
KW - rapeseed
KW - refreshment
KW - regeneration
KW - Solanum tuberosum
U2 - 10.3389/fgeed.2022.780004
DO - 10.3389/fgeed.2022.780004
M3 - Journal article
C2 - 35845346
AN - SCOPUS:85159898064
VL - 4
JO - Frontiers in Genome Editing
JF - Frontiers in Genome Editing
SN - 2673-3439
M1 - 780004
ER -
ID: 351885371