Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast. / Centeno, Francisco; Deschamps, Stéphane; Lompré, Anne Marie; Anger, Marielle; Moutin, Marie Jo; Dupont, Yves; Palmgren, Michael Gjedde; Villalba, Jose Manuel; Møller, Jesper Vuust; Falson, Pierre; le Maire, Marc.

In: FEBS Letters, Vol. 354, No. 1, 31.10.1994, p. 117-122.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Centeno, F, Deschamps, S, Lompré, AM, Anger, M, Moutin, MJ, Dupont, Y, Palmgren, MG, Villalba, JM, Møller, JV, Falson, P & le Maire, M 1994, 'Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast', FEBS Letters, vol. 354, no. 1, pp. 117-122. https://doi.org/10.1016/0014-5793(94)01104-4

APA

Centeno, F., Deschamps, S., Lompré, A. M., Anger, M., Moutin, M. J., Dupont, Y., Palmgren, M. G., Villalba, J. M., Møller, J. V., Falson, P., & le Maire, M. (1994). Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast. FEBS Letters, 354(1), 117-122. https://doi.org/10.1016/0014-5793(94)01104-4

Vancouver

Centeno F, Deschamps S, Lompré AM, Anger M, Moutin MJ, Dupont Y et al. Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast. FEBS Letters. 1994 Oct 31;354(1):117-122. https://doi.org/10.1016/0014-5793(94)01104-4

Author

Centeno, Francisco ; Deschamps, Stéphane ; Lompré, Anne Marie ; Anger, Marielle ; Moutin, Marie Jo ; Dupont, Yves ; Palmgren, Michael Gjedde ; Villalba, Jose Manuel ; Møller, Jesper Vuust ; Falson, Pierre ; le Maire, Marc. / Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast. In: FEBS Letters. 1994 ; Vol. 354, No. 1. pp. 117-122.

Bibtex

@article{e68a470dd7c14fb08c4027710844873e,
title = "Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast",
abstract = "We describe here an easy system for the production of mg amounts of the rabbit Ca2+-ATPase SERCA 1a in the yeast S. cerevisiae. The protein is present in several membranes, including the plasma membrane of the yeast, in a native conformation. It can be purified by immunoprecipitation and can be phosphorylated from ATP in a Ca2+-dependent manner. Using a temperature-sensitive secretion mutant strain, the fully active protein can also be obtained in secretory vesicles.",
author = "Francisco Centeno and St{\'e}phane Deschamps and Lompr{\'e}, {Anne Marie} and Marielle Anger and Moutin, {Marie Jo} and Yves Dupont and Palmgren, {Michael Gjedde} and Villalba, {Jose Manuel} and M{\o}ller, {Jesper Vuust} and Pierre Falson and {le Maire}, Marc",
year = "1994",
month = oct,
day = "31",
doi = "10.1016/0014-5793(94)01104-4",
language = "English",
volume = "354",
pages = "117--122",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - Expression of the sarcoplasmic reticulum Ca2+-ATPase in yeast

AU - Centeno, Francisco

AU - Deschamps, Stéphane

AU - Lompré, Anne Marie

AU - Anger, Marielle

AU - Moutin, Marie Jo

AU - Dupont, Yves

AU - Palmgren, Michael Gjedde

AU - Villalba, Jose Manuel

AU - Møller, Jesper Vuust

AU - Falson, Pierre

AU - le Maire, Marc

PY - 1994/10/31

Y1 - 1994/10/31

N2 - We describe here an easy system for the production of mg amounts of the rabbit Ca2+-ATPase SERCA 1a in the yeast S. cerevisiae. The protein is present in several membranes, including the plasma membrane of the yeast, in a native conformation. It can be purified by immunoprecipitation and can be phosphorylated from ATP in a Ca2+-dependent manner. Using a temperature-sensitive secretion mutant strain, the fully active protein can also be obtained in secretory vesicles.

AB - We describe here an easy system for the production of mg amounts of the rabbit Ca2+-ATPase SERCA 1a in the yeast S. cerevisiae. The protein is present in several membranes, including the plasma membrane of the yeast, in a native conformation. It can be purified by immunoprecipitation and can be phosphorylated from ATP in a Ca2+-dependent manner. Using a temperature-sensitive secretion mutant strain, the fully active protein can also be obtained in secretory vesicles.

UR - http://www.scopus.com/inward/record.url?scp=0028067943&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(94)01104-4

DO - 10.1016/0014-5793(94)01104-4

M3 - Journal article

C2 - 7957891

AN - SCOPUS:0028067943

VL - 354

SP - 117

EP - 122

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1

ER -

ID: 245002833